相关实验视频

Updated: Jul 27, 2025

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

Published on: October 26, 2017

唾液微RNA作为口腔癌的生物标志物

Chameera Ekanayake Weeramange^1,2,3,4, Kai Dun Tang^3,5, Roberto A Barrero⁶

¹Saliva and Liquid Biopsy Translational Laboratory, Griffith Institute for Drug Discovery (GRIDD) and Menzies Health Institute Queensland (MIHQ), Griffith University, Nathan, Queensland, Australia.

Cancer medicine

|June 6, 2023

概括

唾液微RNAs (miRNAs) 在早期口腔癌 (OPC) 检测方面表现有前途. 这项研究确定了用于诊断HPV阳性OPC和预测患者存活率的特定miRNA面板.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物标志物发现发现

背景情况:

口腔癌 (OPC) 的发病率正在上升,特别是与HPV相关的病例.
目前缺乏OPC早期检测方法.
唾液是生物标志物的潜在来源,因为它与头癌有关.

研究的目的:

研究唾液微RNAs (miRNAs) 作为OPC检测的生物标志物.
专注于识别与人类乳头瘤病毒 (HPV) 阳性OPC相关的miRNAs.
评估OPC中的唾液miRNA的诊断和预后潜力.

主要方法:

从OPC患者和对照组收集的唾液样本.
唾液小RNA的下一代测序,以识别失调的miRNA.
在单独的队列中进行定量PCR验证.
一般化后勤回归和生存分析,以评估诊断和预后性能.

主要成果:

一个由九个miRNA组成的小组证明了HPV阳性OPC的高诊断准确性 (AUC>94%).
一个由六个miRNA组成的小组可以将OPC与对照区分开,而不论HPV状态 (AUC>77%).
低调 hsa-miR-7-5p 与较差的 OPC 患者存活率相关.
一个由9个miRNA组成的小组预测了OPC患者的整体存活率.

结论:

唾液miRNAs对于早期检测口腔癌至关重要.
特定的miRNA签名可以帮助诊断OPC和预测患者的预后.
这项研究为OPC的非侵入性诊断和预后工具开辟了道路.

关键词:

生物标志物生物标志物人类乳头瘤病毒人类乳头瘤病毒微型RNA微型RNA是什么这是口腔喉癌.这是唾液,唾液.

更多相关视频

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Published on: May 27, 2016

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...