对后益生菌外聚糖的新方法
Esin Kıray1, Nadia Masser Raheel2
1Department of Medical Services and Techniques, Kırşehir Ahi Evran University Faculty of Medicine, Kırşehir, Turkey
Balkan medical journal
|June 6, 2023
概括
来自Lactobacillus paracasei L1的外聚糖体表现出强烈的抗定数感应和抗菌膜活动. 这些发现表明它们作为抗生素天然替代品的潜力,用于对抗细菌感染.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 生物化学 生物化学
背景情况:
- 越来越多的抗生素耐药性对公众健康构成重大威胁.
- 益生菌微生物及其代谢产品为抗生素提供了潜在的替代品.
- 抑制细菌的定数感应 (QS) 是预防感染的一种策略.
研究的目的:
- 研究来自Lactobacillus paracasei的外聚糖 (EPS) 的QS机制和免疫效应 L1.1.
- 描述EPS的生物和生物化学特性.
- 评估EPS作为抗感染剂的潜力.
主要方法:
- 评估了EPS的抗菌,抗菌膜和抗QS活动.
- 测量了干扰素- (IFN-γ) 和介质素-10 (IL-10) 的产生.
- 总抗氧化能力 (TAC),SEM和GC-MS用于表征.
主要成果:
- L. paracasei L1-EPS对大肠杆菌,金黄色杆菌和P. aeruginosa表现出强烈的抗菌膜活性.
- 在10毫克/毫升EPS时观察到高的抗QS活性.
- EPS显示出免疫刺激作用,增加IFN-γ并降低IL-10的产生.
结论:
- L. paracasei L1-EPS具有显著的抗QS和抗菌膜特性.
- 该EPS表现出抗微生物和抗氧化能力.
- 这些EPS代表了对制药和食品工业应用的有希望的化合物.
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