口腔生物膜冷疗法作为一种新的生态调制方法
N Zayed1,2, J Ghesquière3, N H N Kamarudin4
1Department of Oral Health Sciences, University of Leuven (KU Leuven), Leuven, Belgium.
Journal of dental research
|June 6, 2023
概括
口服冷疗法有效地降低了口服生物膜负载并改变了其结构,选择性地向致病细菌,同时保留有益的细菌. 这种新的方法表明,在没有抗微生物药物的口腔生物膜管理方面有希望.
科学领域:
- 牙科 牙科是指牙科的专业.
- 口腔微生物学 口腔微生物学
- 生物技术是生物技术.
背景情况:
- 口腔冷疗法是一种安全,简单和经济有效的口腔病变的牙科治疗方法.
- 它对口腔生物膜的影响,对口腔健康至关重要,仍然未被探索.
研究的目的:
- 为了研究冷治疗对体外口服生物膜的影响.
- 评估冷治疗对调节口腔生物膜生态学的潜力.
主要方法:
- 在体外多种口腔生物膜建立在交生和异生状态的氧酸盐盘上.
- 生物膜被CryoPen X+处理,并立即或24小时再化后使用CLSM,SEM和v-qPCR进行分析.
主要成果:
- 低温疗法显著降低了生物膜负载,在多个循环后减少的增加.
- 虽然细菌负载在24小时内恢复,但结构和组成的变化仍然存在.
- 与对照组相比,在经过处理的生物膜中,致病物种的发生率显著下降.
结论:
- 喷雾冷疗法为口腔生物膜控制提供了一种新的策略.
- 它选择性地向病原生物,促进共生生物膜状态,并且可能在没有消毒剂/抗菌剂的情况下预防失生症.
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