具有受损CRISPR-Cas防御的Enterococcus faecalis菌株在针对CRISPR向的等离子体的抗生素选择下出现
Wenwen Huo1, Valerie J Price1, Ardalan Sharifi1
1Department of Biological Sciences, The University of Texas at Dallas, Richardson, Texas, USA.
Applied and environmental microbiology
|June 6, 2023
概括
抗生素选择可以削弱Enterococcus faecalis的CRISPR-Cas防御系统,这是导致感染的细菌. 这种妥协提高了它获得新抗生素耐药性等离子体的能力,促进了横向基因转移.
科学领域:
- 微生物学 微生物学
- 细菌遗传学 细菌遗传学
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- Enterococcus faecalis是医院感染的重要原因,也是抗生素耐药性等离子体的载体.
- 耐多药菌株经常含有移动遗传元素 (MGE).
- 在E. faecalis中的CRISPR-Cas系统可以限制MGE获取,但它们的有效性可能是暂时的.
研究的目的:
- 研究抗生素选择对Enterococcus faecalis中的CRISPR-Cas系统的影响.
- 了解CRISPR-Cas功能如何影响抗生素耐药性等离子体的获得.
- 分析CRISPR-Cas系统的动态及其在细菌群体中的目标.
主要方法:
- 在Enterococcus faecalis种群上进行了连续通道实验.
- 深度测序被用来分析种群内的遗传变化.
- 这项研究涉及具有和没有功能CRISPR-Cas系统的人群,在不同的抗生素选择压力下.
主要成果:
- 对等离子体的抗生素选择导致了具有受损CRISPR-Cas防御能力的突变体的出现.
- 这些突变者表现出获得第二种抗生素耐药性等离子体的增强能力.
- 由于没有选择,等离子体从野生型种群中消失,但不是从cas9缺陷突变体中消失.
结论:
- 在抗生素选择下,Enterococcus faecalis CRISPR-Cas系统可能会受到损害.
- 这种妥协方便了横向基因转移和获得额外的耐药性等离子体.
- 了解这些动态对于打击抗生素耐药性传播至关重要.
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