相关实验视频
Updated: Jul 27, 2025
09:40
Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
8.7K
一个小说变体的小说变体
Kieran Bowran1, Stephen R Garrett1, Arnoud H M van Vliet2
1Microbes in Health and Disease Theme, Newcastle University Biosciences Institute, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK.
Microbial genomics
|June 6, 2023
概括
在Listeria monocytogenes中,VIIb型蛋白质分泌系统 (T7SSb) 呈现出显著的多样性,其中新发现的第八种EssC变体和新的LXG-域蛋白质有助于细菌间竞争战略.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 基因组学就是基因组学.
背景情况:
- 类型VIIb蛋白质分泌系统 (T7SSb) 对于Bacillota细菌的细菌间竞争至关重要.
- 作为T7SSb的关键ATPase组成部分,EssC影响基质识别,并表现出序列变异.
- 之前的研究在Listeria monocytogenes中发现了七种EssC变异,与特定的分泌基质有关.
研究的目的:
- 调查T7SSb的多样性,特别是EssC变体和相关蛋白质,在一个大集合的Listeria monocytogenes基因组.
- 确定T7SSb相关蛋白质的新组件和基因组位置,包括LXG域蛋白质.
- 探索Listeria属中EssC类型的流行率和多样性.
主要方法:
- 对37,930个Listeria monocytogenes基因组的生物信息分析.
- 识别和表征EssC序列变异和相邻基因.
- 在不同的Listeria物种中比较基因组学.
主要成果:
- 在Listeria monocytogenes III系中发现了一种罕见的第八种EssC变体 (EssC8),与重组热点 (Rhs) 重复毒素和辅助蛋白有关.
- 确定了九种新的LXG域蛋白质和四个新的染色体热点,用于编码LXG蛋白质.
- 证实所有八种Listeria monocytogenes EssC变种都存在于其他Listeria物种中,并发现了额外的新型EssC类型.
- 观察到Listeria物种经常编码多个EssC类型,突出显示了T7SSb在属内的多样性.
结论:
- 李斯特菌具有高度多样化的T7SSb,其EssC成分和相关的效应蛋白具有显著的变化.
- 鉴定出新的蛋白质和基因组位置扩大了我们对T7SSb功能和进化的理解.
- T7SSb的多样性是Listeria属的一个决定性特征,可能在它们的生态相互作用和病原性中发挥着重要作用.
更多相关视频
相关概念视频
01:05Single Nucleotide Polymorphisms-SNPs
15.3K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
15.3K
01:25Genetic Variation
332
Genetic variation is the diversity in DNA sequences found among individuals of the same species. This diversity is crucial for a species' survival because it helps organisms adapt to environmental changes. Genetic variation begins with fertilization, where an egg and sperm cell merge. Each of these cells carries 23 chromosomes, up to 46 in the fertilized egg. Chromosomes are long DNA strands that contain genes, the basic units of heredity.
Genes exist in different versions called alleles,...
Genes exist in different versions called alleles,...
332
02:26Comparing Copy Number Variations and SNPs
17.8K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
17.8K
00:36Viral Mutations
32.6K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
32.6K
01:29Modern Molecular Taxonomy
56
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
56
01:30Point and Frameshift Mutations
38
Point mutations are genetic alterations involving the change of a single nucleotide base pair in DNA. Depending on how the alteration affects protein synthesis, they can lead to various consequences.Point mutations fall into the following types:Silent mutations occur when a nucleotide change does not alter the amino acid sequence due to the redundancy of the genetic code. For instance, changing ACC to ACA still encodes threonine, leaving the protein function unaffected. This occurs because...
38