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基于亲和的激酶催化交叉链接用于研究激酶-基质对

Rachel J Beltman1, Andrew A Herppich1, Hannah J Bremer1

  • 1Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, Michigan 48202, United States.

Bioconjugate chemistry
|June 6, 2023
PubMed
概括

研究人员开发了新的ATP类似物,ATP-aryl硫酸盐 (AFS) 和ATP-hexanoyl化物 (HexBr),用于在没有紫外线的情况下进行激酶基质交叉连接. ATP-AFS在细胞溶解物中表现出强大的复合物形成和交联,推进了酶相互作用研究.

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Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
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