相关实验视频
Updated: Jul 27, 2025

10:36
Measurements of Physiological Stress Responses in C. Elegans
Published on: May 21, 2020
14.0K
在香皮化过程中,通过WRKY调节器进行低温诱导的调节网络重新布线
Wenjun Zhu1, Hua Li1, Pengfei Dong2
1Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Plant physiology
|June 6, 2023
概括
香皮的色与WRKY转录因子有关. 这些因素调节了参与寒冷耐受性和色途径的基因,为改善水果保质期提供了目标.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 热带水果如香容易受到寒冷的伤害,导致色.
- 了解热带水果的冷反应对于保持质量至关重要.
研究的目的:
- 为了研究香皮中冷诱导的色的分子机制.
- 描述基因调节的变化,染色质的可访问性和在寒冷压力下转录因子的结合.
主要方法:
- 染色质可访问性,基因组修饰,cis调节元件,转录因子结合和基因表达的系统性表征.
- 用DNA亲和力净化测序,光酶记者测试和短暂表达测试来验证研究结果.
主要成果:
- 香皮中的冷诱导转录显示了染色质可访问性和基因组修饰的协调变化.
- 对WRKY结合部位进行了升级调节的基因,寒冷特别诱导香WRKYs.
- 通过WRKYs调解增强剂-促进剂相互作用,调节色和耐寒路径.
结论:
- 由WRKY转录因子调解的广泛的转录重编程导致香皮在寒冷压力下变棕.
- 研究结果提供了对热带植物寒冷压力基因调节的见解.
- 已识别的WRKY和途径为提高热带水果的耐寒性和保质期提供了潜在的目标.
相关概念视频
Responses to Heat and Cold Stress
13.6K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.6K
Regulation of the Unfolded Protein Response
2.5K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.5K
Gene Regulation During Sporulation
42
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
42
Cell Signaling in Plants
5.7K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.7K
Riboswitches
8.2K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.2K
Regulation of Transpiration by Stomata
28.5K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
28.5K

