基于微观卫星分析的中国和越南本地品种的遗传多样性和品种鉴定
Zhong Zhuang1, Long Zhao1, Weicheng Zong2
1Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Journal of animal science
|June 6, 2023
概括
这项研究使用微观卫星标记器分析了来自中国和越南的21种当地品种的遗传多样性. 结果显示丰富的遗传资源和密切的关系,有助于保护这些独特的家禽种群的保护工作.
科学领域:
- 动物遗传学和动物基因组学
- 保护生物学 保护生物学
- 禽畜科学 禽畜科学 禽畜科学
背景情况:
- 南亚和东南亚是家的来源中心,拥有丰富的家禽遗传资源和独特的当地品种.
- 全球禽畜养殖的强化威胁到许多当地品种的灭绝.
- 在中国和越南,保护和繁殖本地品种至关重要,尤其是在中国"一带一路"政策的背景下.
研究的目的:
- 描述中国南部和越南当地品种的遗传多样性和种群结构.
- 开发微卫星分子遗传标记,用于品种识别和保护.
- 为改善品种保护和生殖质构造提供见解.
主要方法:
- 对来自中国南部 (云南,广西) 和越南的21个当地种群的分析.
- 18个微卫星分子遗传标记物的表征.
- 构建微卫星位置的品种识别标签.
- 对遗传多样性,多态信息含量 (PIC),异构卵子过量,遗传差异化 (FST) 和遗传距离 (DS) 的分析.
- 使用邻近连接的树枝图和使用STRUCTURE程序进行人口结构分析的家族遗传学分析.
主要成果:
- 总共检测到377个等位基因,其中LEI0094位点显示的等位基因数量最多 (44) 和PIC (0.7820).
- 平均PIC值为0.65,表示中度多态性和丰富的基因多样性跨种群.
- 观察到高度的遗传差异化,越南品种之间表现出较低的FST和DS.
- 聚类分析显示,中国南部和越南品种之间存在密切的遗传关系,但除了东陶这样的例外.
- 为20种品种开发了独特的微卫星标记器,使用了15个微卫星位置.
结论:
- 研究的种群在遗传上很丰富,由于地理接近和人类活动,它们之间关系密切.
- 这些发现支持中国和越南当地品种的保护和繁殖.
- 东,中国云南当地品种 (Gallus gallus spadiceus) 和红色林 (Gallus gallus) 的潜在共同起源被建议.
相关概念视频
00:49Complementation Tests
5.0K
A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by Edward Lewis in the 1940s while working on fruit flies. He developed the test to identify the location and arrangement of different mutations on chromosomes.
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
5.0K
01:29Modern Molecular Taxonomy
56
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
56
02:26Comparing Copy Number Variations and SNPs
17.8K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
17.8K