双电位编码的电化学发光用于多重基因测定,其中一个光作为标签
Xuwen Gao1, Xiaoxuan Ren1, Yaojia Ai1
1School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, PR China.
Biosensors & bioelectronics
|June 6, 2023
概括
这项研究引入了一种新的双电位电化学发光 (ECL) 试验,用于多重基因检测. 这种无核活性剂的方法可以同时检测多个核酸标,以准确诊断疾病.
科学领域:
- 分析化学 分析化学
- 纳米技术 纳米技术
- 生物医学诊断 生物医学诊断
背景情况:
- 目前的体外诊断 (IVD) 基因测试仅限于单个目标检测.
- 多复合基因测试对于准确的疾病诊断和预测至关重要.
- 需要先进的方法来同时检测多个核酸标.
研究的目的:
- 为多重基因测定开发一种双电位编码的,无核活性剂的电化学发光 (ECL) 策略.
- 为了使用单个发光标签同时检测多个核酸目标.
- 为了证明这种策略用于检测特定病毒基因的可行性.
主要方法:
- 使用双稳定剂覆盖的 Telluride (CdTe) 纳米晶体 (NCs) 作为发光标签.
- 使用不同的链接策略 (Cd-S键和胺链接) 实现不同的电化学潜力,与RNA功能化的CdTeNCs.
- 使用电化学发光 (ECL) 来检测功能化的CdTeNCs.
主要成果:
- 获得的潜在选择性ECL信号:一种是硫-RNA功能化的CdTeNCs的~0.32V,另一种是氨基-RNA功能化的CdTeNCs的~0.82V.
- 成功证明了对开放的读取框架1ab (ORF1ab) 和核蛋白 (N) 基因的多重检测,没有交叉.
- 两种基因的确定的低检测极限 (LOD):ORF1ab的100aM和N基因的2fM.
结论:
- 拟议的双潜力ECL策略为多重基因测定提供了一种新的方法.
- 通过受控标签-键工程通过RNA进行后工程CdTeNCs提供了潜在选择性和编码的ECL检测方法.
- 该方法使用单个光进行同时,灵敏和特定检测多个核酸标.
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