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由Bacillus brevis 1B从受污染的环境中降解以乙酶和ALDH脱酶为基础的农药降解

Saurabh Gangola¹, Geeta Bhandari², Samiksha Joshi¹

¹School of Agriculture, Graphic Era Hill University, Bhimtal, 263136, India.

Environmental research

|June 6, 2023

概括

巴西菌株1B有效地生物修复了一种农药混合物,在15天内降解高达95%. 这种细菌菌株显示出从杀虫剂和其他毒素中清理受污染的场所的潜力.

科学领域:

环境微生物学环境微生物学
生物修复技术生物修复技术
杀虫剂降解降解方法

背景情况:

农药污染对环境和健康构成重大风险.
制定有效的生物修复策略对于环境清洁至关重要.
巴西菌株1B因其潜在的杀虫剂降解能力而被分离出来.

研究的目的:

评估Bacillus brevis菌株1B在降解常见农药混合物的有效性.
通过菌株1B.确定农药生物修复的最佳条件.
为了识别与降解过程有关的中间代谢物和基因.

主要方法:

隔离和表征Bacillus brevis菌株1B. 的情况.
确定可容忍的最大杀虫剂度.
使用响应表面方法 (RSM) 优化生物修复条件.
基于土壤的生物修复实验和使用气体染色学-质谱学 (GC-MS) 的分析.

主要成果:

巴西菌株1B可以耐受450毫克L-1的伊米达克洛普里德,菲普罗尼尔,赛珀米特林和硫硫.
菌株1B在15天内在最小的介质中实现了杀虫剂混合物 (20毫克L-1) 的高达95%的降解.
生物修复的最佳条件包括2.0 × 107 CFU mL-1的注射液,每分钟120转的速度,以及80 mg L-1的农药度.
土壤生物修复显示了高降解率:99%的伊米达克洛普里德,98.5%的菲普罗尼尔,94%的甲和91.67%的硫硫.
在GC-MS分析中,发现了几种甲氨酸降解的中间代谢产物.
在压力条件下观察到脱酶 (ALDH) 和酶基因的表达.

结论:

巴西虫菌株1B在降解农药混合物方面表现出高效.
该研究确定了最佳条件和参与生物修复过程的关键基因.
这种细菌菌株对受农药污染的场所和其他有毒物质的生物修复有希望.

关键词:

据报道,阿尔德 (ALDH) 已经开始.巴西菌 1B 1B 1B 1B 1B 生物降解生物降解埃斯特拉斯 (Esterase) 是一种酶.中间代谢产物中间代谢产物杀虫剂是一种杀虫剂.

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