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土壤太阳化加上肥料修改对夏季休期间土壤ARG和微生物群落的影响

Xiaojuan Wang¹, Li Zhang¹, Jie Gu²

¹College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, 712100, China.

Environmental pollution (Barking, Essex : 1987)

|June 6, 2023

概括

土壤太阳化与有机修改相结合,在夏季休期间在温室蔬菜生产土壤中减少了抗生素耐药性基因 (ARG) 和移动遗传元素 (MGE). 环境因素和水平基因转移影响了ARG的丰富性,特定的细菌被确定为潜在的宿主.

科学领域:

农业科学农业科学
环境微生物学环境微生物学
土壤科学土壤科学

背景情况:

土壤太阳化 (SS) 通过增加温度来管理土壤病原体和杂草.
肥会改变土壤细菌群落,需要进行有机修改以提高效果.
有机修改可以将抗生素耐药性基因 (ARG) 引入农业土壤.

研究的目的:

调查SS与不同有机修改对夏季休期间温室蔬菜生产 (GVP) 土壤中ARG和移动遗传元素 (MGE) 的影响.
确定影响ARG动态和潜在的细菌宿主在GVP土壤中的关键因素.

主要方法:

使用高通量PCR (qPCR) 来量化ARG和MGE.
分析了在SF期间不同肥和SS处理下GVP的土壤样本.
使用网络分析来确定细菌宿主和ARG之间的相关性.

主要成果:

在SF期间,用肥料和SS处理的GVP土壤中,ARG和MGE的丰度和多样性下降.
由酸盐和等环境因素驱动的水平基因转移,特别是通过整合酶,是ARG变化的主要驱动因素.
蛋白质细菌和菌被确定为主要的ARG宿主,特定的属与某些耐药性基因相关.

结论:

将SS与有机修订物相结合,可以有效地减少SF期间GVP土壤中的ARGs和MGEs.
了解ARG命运和转移机制对于减轻它们在农业系统中的传播至关重要.
这项研究为可持续的GVP土壤管理提供了见解,以减少抗生素耐药性的环境负担.

关键词:

抗生素耐药性基因是抗生素耐药性的基因.细菌社区的细菌社区温室蔬菜生产土壤温室蔬菜生产土壤经过修改的肥料.土壤太阳化土壤的太阳化.

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