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欧核生物和巨型病毒共享的一组新型的胺相关蛋白质能够从矩阵内部重塑线粒体

Shaghayegh Sheikh^1,2, Tomáš Pánek³, Ondřej Gahura¹

¹Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic.

Molecular biology and evolution

|June 6, 2023

概括

研究人员在巨型病毒和真核生物中发现了一种新型的胺相关蛋白 (DRP) 家族,MidX. MidX独特地从内部重塑线粒体,表明横向基因转移并挑战以前的DRP同质假设.

科学领域:

细胞生物学细胞生物学
病毒学病毒学
进化生物学是进化的生物学.

背景情况:

动氨酸相关蛋白质 (DRPs) 对细胞过程至关重要,包括由Mgm1 (真菌) 和Opa1 (元生动物) 进行线粒体内膜重塑.
以前对DRP的理解仅限于已知的真核细胞群.

研究的目的:

在多种真核生物和病毒基因组中识别和描述新型DRP.
为了研究一个新发现的DRP类,MidX.的功能和进化起源.

主要方法:

对DRP进行详尽的基因组和元基因组数据库搜索.
遗传学分析以确定DRP关系.
在Trypanosoma brucei中对Hyperionvirus MidX的外源表达,以研究其对线粒体的影响.

主要成果:

在真核生物和巨型病毒 (Nucleocytoviricota) 中发现了新的 DRP 类型,包括 MidX 类.
预计MidX蛋白是针对线粒体的,并且具有独特的三级结构.
在Trypanosoma brucei中MidX的外源表达导致了线粒体形态从母体内显著改变,与内膜密切相关.
遗传学分析将Mgm1作为MidX的姊妹群,而不是Opa1.

结论:

MidX代表了一个新的DRP家族,具有前所未有的线粒体内膜从矩阵重塑的机制.
巨型病毒可能通过从真核生物的水平基因转移获得了MidX,以便在感染期间操纵宿主线粒体.
MidX的独特结构和功能表明,它具有针对病毒与宿主相互作用的特殊适应性.
MidX的基因位置挑战了Mgm1和Opa1.1之间的假定同质性.

关键词:

核细胞核细胞病毒 Mgm1Mgm1Mgm1Mgm1Mgm1Mgm1Mgm1Mgm1Mgm1Mgm1Mgm1Mgm1Mgm1Mgm1Mgm1Mgm1Mgm1 在Opa1中,Opa1是指Opa1的意思.迪纳明超级家族的超级家族线粒体中的线粒体.原始主义者是原始主义者.

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