相关实验视频

Updated: Jul 17, 2026

In vitro Cell Culture Model for Toxic Inhaled Chemical Testing

In vitro Cell Culture Model for Toxic Inhaled Chemical Testing

Published on: May 8, 2014

在实验室中评估老鼠和人类呼吸道上皮模型,用于急性吸入毒性测试

Joanne Wallace¹, George R Jackson², Yulia Kaluzhny²

¹Charles River Laboratories Edinburgh Ltd, Elphinstone Research Centre, Tranent, East Lothian EH33 2NE, UK.

Toxicological sciences : an official journal of the Society of Toxicology

|June 6, 2023

概括

一个新的实验室大鼠气道上皮模型 (RAEM) 显示了与人类模型相似的毒性反应. 这种经过验证的老鼠EpiAirway模型有助于预测体内老鼠毒性,并支持3Rs倡议.

科学领域:

毒理学毒理学毒理学
在体外模型模型.
呼吸道毒理学呼吸道毒理学

背景情况:

在体内动物模型是急性吸入毒性评估的标准.
作为替代方案,正在开发人体呼吸道上皮层模型 (HAEM).
毒性反应的物种间变异性需要比较模型.

研究的目的:

开发和描述一个器官类型的实验室大鼠气道表皮模型 (RAEM),大鼠EpiAirway.
直接比较RAEM与人类的经气道 (HAEM),以评估物种间的变异性.
验证RAEM用于预测体内老鼠毒性,并支持3Rs程序.

主要方法:

在两个独立的实验室中评估了大鼠的经气道 (RAEM) 和人类的经气道 (HAEM).
使用了14种具有已知的毒性概况的参考化学品.
评估的毒性终点:组织活力 (MTT),屏障完整性 (TEER) 和组织病理学.

主要成果:

鼠的EpiAirway模型在实验和实验室中证明了可重现的结果.
观察到RAEM和HAEM毒性反应之间的高度一致性 (R2值高达0.92).
鼠和人类的气道模型对急性化学物质暴露的反应相似.

结论:

在体外开发的RAEM为毒性测试提供了可靠的替代方案.
鼠和人类的呼吸道上皮组织对急性化学伤害的反应相似.
这种RAEM有助于推断到体内老鼠毒性,并有助于对3Rs进行化学查.

关键词:

在体外呼吸道模型.吸入性毒性测试吸入性毒性测试鼠的气道模型

更多相关视频

Assessment of the Acute Inhalation Toxicity of Airborne Particles by Exposing Cultivated Human Lung Cells at the Air-Liquid Interface

Assessment of the Acute Inhalation Toxicity of Airborne Particles by Exposing Cultivated Human Lung Cells at the Air-Liquid Interface

Published on: February 23, 2020

An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing

An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing

Published on: May 13, 2020

相关概念视频

Toxicity Testing in Animals

Toxicity Testing in Animals

Toxicity tests in animals are grounded on two main assumptions: first, the effects observed in laboratory animals can be extrapolated to humans, especially when adjusted for body surface area; second, high-dose exposure in animals is essential to identify potential human hazards from lower doses. This is based on the quantal dose-response concept, which faces the challenge of extrapolating results from relatively few test animals to much larger human populations. For example, a 0.01% incidence...