无间隙的基因组组合的异体鼻 (Coilia nasus)
Fengjiao Ma1, Yinping Wang1,2, Bixiu Su3
1Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China.
Scientific data
|June 6, 2023
概括
研究人员已经为中国尾鱼 (Coilia nasus) 创建了一个无缺口的染色体级基因组. 这种高质量的参考基因组将促进对其基因组结构的理解,并有助于保护工作.
科学领域:
- 基因组学就是基因组学.
- 水生生物学 水生生物学
- 渔业 科学 渔业 科学
背景情况:
- 中国尾鱼 (Coilia nasus) 是一个重要的无鱼物种.
- 之前的C. nasus参考基因组含有空白,限制了基因组分析.
- 了解基因组架构对于保护和管理至关重要.
研究的目的:
- 组装一个高质量的,染色体水平,Coilia nasus的无缺口参考基因组.
- 为了克服以前的缺口基因组组合所带来的局限性.
- 为未来的研究提供一个强大的基因组资源.
主要方法:
- 纳入高覆盖率,准确的长读序列数据.
- 应用多种先进的基因组组装策略.
- 功能性基因注释使用de novo预测,蛋白质同类学和RNA-seq数据.
主要成果:
- 一个完整的,没有空白的,染色体水平的基因组组合,用于C. nasus,包括24个染色体.
- 基因组大小为851.67 Mb,估计完整度为92.5% (BUSCO).
- 21,900个基因的功能注释,覆盖了预测的99.68%的蛋白质编码基因.
结论:
- 新组装的无缺口基因组代表了迄今为止C. nasus.的最高质量和完整性.
- 这种资源可以对基因组结构和功能进行深入研究.
- 它为这种经济重要物种的有效管理和保护战略提供了关键的基础.
相关概念视频
Genome Annotation and Assembly
18.9K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.9K
Multi-species Conserved Sequences
4.0K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
4.0K
Cis-regulatory Sequences
3.0K
3.0K
Lampbrush Chromosomes
7.9K
In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
7.9K
Oligosaccharide Assembly
2.9K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
2.9K


