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相关的生物组织的冷-SEM和CryoNanoSIMS成像

Anders Meibom^1,2, Florent Plane^3,4, Tian Cheng³

¹Laboratory for Biological Geochemistry, School of Architecture, Civil and Environmental Engineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, CH-1015, Switzerland. anders.meibom@epfl.ch.

|June 6, 2023

概括

一个新的CryoNanoSIMS仪器在同位素分析过程中保留了生物组织形态和可溶性化合物. 这种冷工作流可以为先进的生物研究提供原始的亚细胞化学和同位素映射.

科学领域:

生物物理学的生物物理.
分析化学分析化学
细胞生物学细胞生物学

背景情况:

纳米级二次离子质谱 (NanoSIMS) 提供生物组织的高分辨率分析.
目前的NanoSIMS样品制备导致组织扭曲和可溶性化合物的损失.
需要一个冷工作流程来保持组织完整性,以便进行准确的分析.

研究的目的:

为NanoSIMS.开发一个冷样品准备和成像工作流程.
为了使玻璃化生物组织的高分辨率同位素和元素映射.
为了克服传统NanoSIMS样品准备的局限性.

主要方法:

开发一个CryoNanoSIMS仪器.
通过高压结进行玻璃化.
低温绘制和低温扫描电子显微镜 (cryo-SEM) 成像.
阳性和阴性二次离子的同位素成像.

主要成果:

CryoNanoSIMS实现了与传统NanoSIMS相比的质量和图像分辨率.
在Green Hydra组织中展示了同位素和微量元素映射.
在玻璃化样本中保存组织形态和可溶性化合物.

结论:

CryoNanoSIMS能够实现相关的超结构和同位素/元素成像.
工作流程保持生物组织在最原始的死后状态.
开辟了研究基本细胞和组织水平过程的新途径.

关键词:

高压结高压结水是非常有魅力的水.同位素标签的标记这是一个纳米SIMS.透调节是指透调节的过程.光合作生态 (Photosymbiosis) 是一种

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