阿普塔默功能化核酸纳米药物用于结肠癌的向协同疗法
Liye Zhu1,2, Jieyu Yuhan1,3, Hao Yu1,3
1Food Laboratory of Zhongyuan, Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, No. 17 Qinghua Donglu, Beijing, 100083, China.
Journal of nanobiotechnology
|June 6, 2023
概括
这项研究开发了一种针对结肠癌的新型纳米海绵药物递送系统,将向RNA和化疗结合起来,通过诱导亡和细胞循环停止来有效地杀死癌细胞.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术 纳米技术
- 在瘤学瘤学.
背景情况:
- 结肠癌由于其病理生理学,转移和不良预后,提出了复杂的挑战,需要组合疗法.
- 目前的治疗策略需要提高向性治疗和疗效.
研究的目的:
- 开发一种功能性核酸纳米海绵药物 (FND) 输送系统,用于向结肠癌治疗.
- 研究FND系统的抗瘤机制,包括其对细胞活力,细胞亡,细胞循环,ROS和线粒体膜潜力的影响.
主要方法:
- 利用滚动圆转录 (RCT) 来制造AS1411@antimiR-21@Dox纳米海绵系统.
- 采用AS1411吸收酶,对结肠癌细胞进行有针对性的输送.
- 进行了全面的体外分析,包括细胞活力,亡试验,细胞周期分析,ROS量化和线粒体膜潜能测量.
- 进行了转录基因分析,以阐明抗瘤机制.
主要成果:
- 该FND系统证明了有效的向向癌细胞传递.
- 分析证实了FND通过降低细胞活力,增加细胞亡,导致细胞循环停止,改变ROS水平和影响线粒体膜潜力来诱导癌细胞死亡的能力.
- 转录基因数据显示,FND的抗瘤作用通过涉及细胞循环调节 (线粒转移期和亚相) 和亡诱导 (IAP:caspase复合体的SMAC介导解离) 的途径进行介导.
结论:
- 开发的纳米协同治疗系统使得RNA和化疗剂在结肠癌治疗中具有智能和有效的有针对性的联合递送.
- 该FND系统触发细胞循环停止和细胞亡,为结肠癌治疗提供一种可定制,有针对性,可靠,稳定和负担得起的方法,具有高有效负载效率.
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