与ceftiofur相比,cefquinome对猪肠道微生物群和抗体产生更大的影响
Sofie Rutjens1, Nick Vereecke2,3, Jannes Sauer2
1Department of Pathobiology, Pharmacology and Zoological Medicine, Laboratory of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ghent University, 9820, Merelbeke, Belgium.
Veterinary research
|June 6, 2023
概括
塞法洛斯波林治疗 (ceftiofur, cefquinome) 改变了猪便的微生物组和抗体组,增加了蛋白质细菌. 治疗两种抗生素后21天,耐药性基因水平恢复到基线水平.
科学领域:
- 兽医微生物学 兽医微生物学
- 抗微生物耐药性 抗微生物耐药性
- 肠道微生物组研究研究
背景情况:
- 类对于治疗动物严重的细菌感染至关重要.
- 人们对它们对肠道微生物群的影响以及抗菌素耐药性基因的传播存在担忧.
- 了解这些对猪的影响对于负责任的抗生素使用至关重要.
研究的目的:
- 调查塞夫季奥弗和塞夫对猪便微生物组和抵抗体的影响.
- 分析微生物组成的变化和常规肌内治疗后耐药基因的流行情况.
- 为优化猪类抗微生物治疗策略提供见解.
主要方法:
- 长期阅读的16S rRNA基因测序和枪头元基因组测序被采用.
- 便样本从多个时间点的猪中采集,这些猪接受了Ceftiofur,Cefquinome或对照组的治疗.
- 分析的重点是微生物多样性,组成和抗菌素耐药性基因丰富性的变化.
主要成果:
- 塞夫蒂奥弗治疗增加了蛋白质细菌,并为特定的耐药基因 (TetQ,CfxA6,blaTEM-1) 进行了选择.
- Cefquinome 减少了物种丰富性,增加了蛋白质细菌,并且明显影响了更多的属比 ceftiofur.
- 此外,Cefquinome还增加了6个抗菌素耐药性基因,与特定属没有明确的联系;对两者来说,耐药性在治疗后21天正常化.
- 塞夫基诺姆治疗显著影响了18个属,而塞夫基诺姆治疗显著影响了18个属,而塞夫基诺姆治疗显著影响了18个属,而塞夫基诺姆治疗显著影响了18个属.
结论:
- 传统的肌肉内塞法洛斯波林使用会改变猪肠道微生物组和抵抗组.
- 特定的头类会诱导微生物组成和耐药性基因配置文件的明显变化.
- 治疗后21天内,耐药水平恢复到基线,这表明暂时的影响.
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