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在辅助剂诱导的关节炎模型中,增加的肠道透性和肠道炎症在关节炎发作之前
Sophie Hecquet1,2, Perle Totoson1, Hélène Martin1
1Université de Franche-Comté, PEPITE, 25000, Besançon, France.
Arthritis research & therapy
|June 6, 2023
概括
在大鼠模型中,炎症和失生症等肠道变化先于关节炎的发展. 然而,这些肠道变化与疾病的发展过程并非完全不可分割.
科学领域:
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
背景情况:
- 脊椎关节炎与肠道炎症,失生症,增加肠道透性 (IP) 和细菌转移 (BT) 有关.
- 这些肠道变化在疾病发病过程中的确切时间和作用仍在争论中.
研究的目的:
- 在辅助剂诱导性关节炎 (AIA) 鼠标模型中研究肠炎,IP,微生物群变化和BT之间的时间关系.
- 了解与关节炎发展相关的肠道事件的序列.
主要方法:
- 辅助剂诱导性关节炎 (AIA) 的老鼠在临床前 (第4天),发病 (第11天) 和急性 (第28天) 阶段被分析.
- 肠道透性通过佐努林和iFABP水平进行评估.
- 肠道炎症通过淋巴细胞计数和亲炎性细胞因子mRNA表达来评估.
- 使用LPS,可溶性CD14和16SrRNA测序分析了细菌转位和微生物群组成.
主要成果:
- 在AIA大鼠中,在不同阶段观察到血佐努林和iFABP水平的升高.
- 在临床前和发病阶段发生过渡性肠道失生症和升高的促炎性细胞因子mRNA (IL-8,IL-33,IL-17,TNF-α,IL-23p19).
- 在早期阶段,在阴茎中发现了CD4+和CD8+T细胞的增加;没有检测到显著的细菌转移.
结论:
- 在AIA模型中,肠道变化,包括炎症和失生症,在关节炎临床发病之前发生.
- 这些发现表明,虽然肠道变化在关节炎之前就开始了,但它们可能与疾病过程并不完全密不可分.
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