在消化前和消化期间,对新胚胎 (Cynops pyrrhogaster) 的潜在内皮细胞细胞形态和细胞外基质变化的超结构研究: (两动物-新/潜在内皮细胞/形态变化/细胞外基质)
1Department of Anatomy, Saitama Medical School, Morohongo, Moroyama-cho, Iruma-gun, Saitama 350-04 Japan.
Development, growth & differentiation
|June 7, 2023
概括
新胚胎的内部细胞在胃化过程中增加了细胞间接触和细胞外基质 (ECM). 空间中的糖原颗粒可能有助于细胞迁移.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 胚胎学 胚胎学
背景情况:
- 了解早期胚胎发育需要检查细胞行为和细胞外基质 (ECM).
- 从毛囊转向胃囊的过渡涉及显著的细胞重组和相互作用.
研究的目的:
- 为了研究细胞形态,细胞间接触,以及新胚胎内细胞的ECM.
- 为了将ECM的变化与胃流动期间的细胞行为相关联.
主要方法:
- 使用光和电子显微镜检查新 (Cynops pyrrhogaster) 胚胎.
- 蓝和酸染色确定了ECM组件. 蓝和酸染色确定了ECM组件.
- 分析了细胞形态和细胞间空间,从毛囊到胃囊阶段进行分析.
主要成果:
- 内细胞表现出细胞对细胞接触的增加,并形成了斑块,filopodia和lamellipodia.
- 细胞表面的细胞外基质 (ECM) 组成部分从芽细胞到胃细胞阶段增加.
- 在细胞间空间中观察到类似糖原的颗粒,可能有助于细胞迁移.
结论:
- 增加的ECM可能与细胞间接触的改变以及新胚胎发育过程中的过程形成有关.
- 糖原颗粒可能在化过程中为迁移细胞维持细胞间空间发挥作用.
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