在Ca的作用
1Department of Biology, School of Education, Waseda University, Nishiwaseda, Shinjuku-ku, Tokyo 169-50, and Teikyo Junior College, Honcho, Shibuya-ku, Tokyo 151, Japan.
Development, growth & differentiation
|June 7, 2023
概括
对于星卵泡细胞产生1-甲基 (1-MeAde) 作为对生殖腺刺激物质 (GSS) 的反应至关重要. 除去会显著降低循环AMP (cAMP) 水平,阻碍1-MeAde的产生,并表明GSS可能不会结合它的受体.
科学领域:
- 海洋生物学 海洋生物学
- 生殖内分泌学 生殖内分泌学
- 细胞信号传递 细胞信号传递
背景情况:
- 海星卵泡细胞通过生产1-甲基氨酸 (1-MeAde) 来调解卵细胞的成熟.
- 性腺刺激物质 (GSS) 是一种关键的激素,它调节了海星中的1-MeAde生产.
- 细胞外 (Ca2+) 在海星卵泡细胞中GSS介导的信号传递中的作用仍然不完全理解.
研究的目的:
- 研究无海水 (CaFSW) 处理对海星毛囊细胞1-MeAde产生的影响.
- 阐明细胞外在GSS诱导的循环AMP (cAMP) 生产和随后的1-MeAde合成中的作用.
- 探索耗对海星卵泡细胞中G蛋白结合受体信号通路的潜在影响.
主要方法:
- 星毛囊细胞被 Ca2 + 免费海水 (CaFSW) 处理.
- 测量了1-甲基 (1-MeAde) 和循环AMP (cAMP) 的产生,以应对性腺刺激物质 (GSS).
- 使用[α-32P]NAD+与霍乱和百日咳毒素进行的ADP-ribosylation测定是在膜制剂上进行的.
主要成果:
- 在应对GSS时,CaFSW治疗显著降低了1-MeAde的产生.
- 用CaFSW处理的细胞中的cAMP水平较低,并且只显示了GSS的微小增加,不足以产生1-MeAde.
- 1-MeAde和cAMP的产生取决于细胞外Ca2+度,显著降低到1mM Ca2+以下.
- ADP-ribosylation揭示了刺激性和抑制性G蛋白的存在,但adenylyl cyclase活性不受GSS的影响.
结论:
- 在 CaFSW 处理的海星毛囊细胞中减少的 1-MeAde 生产是由于cAMP水平的降低.
- 细胞外对于有效的GSS信号传输至关重要,可能是通过促进GSS受体结合或下游cAMP生产.
- 这些发现表明,在用CaFSW冲洗后,GSS可能无法与毛囊细胞中的受体结合,从而影响信号级联.
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