在储存过程中孤立的线索器官的稳定性
1Biology Department, York University, Downsview, Ontario M3J 1P3, Canada.
Development, growth & differentiation
|June 7, 2023
概括
研究人员使用不同的方法分离了海的线粒体器官 (MA). 乙烯甘醇分离影响了MA的双折稳定性和衰变,而甘二甲基硫氧化物提供了更好的稳定性.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 发展生物学 发展生物学
背景情况:
- 线性器官 (MA) 对于细胞分裂至关重要.
- 了解MA的物理性质,如双折射,可以提供对其结构和功能的见解.
研究的目的:
- 为了比较使用不同程序分离的线粒体器官 (MA) 的特性.
- 调查影响单一MA突破及其稳定性的因素.
主要方法:
- 通过使用烯基醇和甘二甲氧化物 (MTME) 方法,从海的胞中分离了线性器官 (MA).
- 在不同的pH条件下和用H2O或0.5M KCl.提取后测量和比较MA双断率.
- 对双断裂衰变速率和随时间的稳定性的分析.
主要成果:
- 与乙烯甘醇分离的MA表现出pH依赖的双折射,pH较低会增加双折射和稳定性.
- 用甘二甲基硫氧化物 (MTME) 隔离的MA显示出显著更大的双断裂稳定性,衰变速率大约比用烯基醇隔离的1000倍慢.
- 提取后的残留双断率取决于隔离方法,储存介质和储存时间.
结论:
- 隔离方法显著影响了线粒体器官 (MA) 的双折特性和稳定性.
- 甘油二甲基硫氧化物 (MTME) 是一种优质的隔离介质,可以保持MA双断层稳定性.
- 多个组成部分,可能包括定向核糖体,可能有助于单独的MA的双断裂.
更多相关视频
11:08Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae
Published on: October 16, 2014
12.6K
12:04Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
Published on: June 24, 2019
9.6K
相关概念视频
Microtubule Instability
5.2K
Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated...
5.2K
Meiosis vs. Mitosis
57.2K
Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
57.2K
Meiosis II
183.7K
Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each...
183.7K
