在海蛋中注射的cylactin-β-galactosidase融合基因表达的空间模式
Randall Collura1, Karen S Katula1
1Department of Biology West Virginia University Morgantown, West Virginia 26505.
Development, growth & differentiation
|June 7, 2023
概括
海 ?? Cyl actin基因表达与细胞分裂有关. 控制这种空间模式的调节序列位于基因或其第一个内基因上游的前195个基因对内.
科学领域:
- 发展生物学 发展生物学
- 分子遗传学 分子遗传学
- 海洋生物学 海洋生物学
背景情况:
- 海 (Strongylocentrotus purpuratus) 中的Cylactin基因显示出与细胞分裂相关的表达模式.
- 纤维活蛋白转录最初存在于所有早期的芽细胞中,但随着细胞退出细胞循环,它们从胎儿外皮中消失.
研究的目的:
- 为了研究海Cylactin基因的转录调节.
- 确定负责Cyl actin.空间表达模式的调节序列.
主要方法:
- 在受精的海 (Lytechinus pictus) 蛋中微注射各种Cyl-β-gal融合基因.
- 对记者基因结构的空间表达模式的分析.
- 上游序列的删除分析和保存的监管元素的突变.
主要成果:
- 一个具有2.5kb上游序列的Cyl-β-gal融合基因复制了内源Cylactin表达模式.
- 将上游序列删除到 -195 bp 减少了染色,但保持了类似的空间模式.
- 保存的血清反应元素和相邻的结合部位的突变没有改变腹腔外皮染色或CAT活性.
结论:
- 对于Cyl actin空间表达的关键调节序列位于转录起始点和/或第一个内部的第一个195 bp上游.
- 测试的血清反应元素和相邻的结合部位不仅仅负责限制Cylactin在胎儿外皮中的表达.
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