相关实验视频
Updated: Aug 13, 2026
05:46
Isolation and In vitro Activation of Caenorhabditis elegans Sperm
Published on: January 31, 2011
在受精的新蛋中抑制辅助精子的实验*: (多精子/裂变/精子核/新蛋)
Yasuhiro Iwao1, Hisashi Yamasaki1, Chiaki Katagiri2
1Department of Biology, Faculty of Science, Yamaguchi University, Yamaguchi 753, Japan.
Development, growth & differentiation
|June 7, 2023
概括
新蛋中的辅助精子形成前核,但退化. 胚胎细胞核抑制了辅助精子在分裂中的参与,很可能是通过抑制中心角复制,防止适当的细胞分裂.
科学领域:
- 发育生物学是发展生物学.
- 细胞生物学 细胞生物学
- 生殖生物学 生殖生物学
背景情况:
- 新蛋 (Cynops pyrrhogaster) 是多精子的,这意味着多个精子可以受精于一个卵子.
- 这些卵子中的辅助精子启动了前核形成和DNA合成,但通常会退化.
- 这些辅助精子的命运以及它们在早期发育中的作用尚未完全被理解.
研究的目的:
- 为了研究控制多种精子新蛋中辅助精子命运的因素.
- 为了确定胚胎细胞核对辅助精子行为和裂变的影响.
- 阐明早期胚胎发育过程中辅助精子抑制的基础机制.
主要方法:
- 微处理受精的新蛋,包括切割以创建merogons.
- 使用循环赫西米德抑制核事件,使用阿菲迪科林进行DNA合成.
- 在早期分裂阶段对核和细胞质事件的细胞学检查.
主要成果:
- 安德罗梅罗基子 (含有精子前核的卵半部分) 在胚胎细胞核形成之前产生时经常分裂,但在胚胎细胞核形成后不会发生分裂.
- 配件精子在被高细胞质桥分离离离二倍体美龙时未能参与裂变.
- 卵核的去除或早期核事件的抑制诱导多极裂变.
- 缺少DNA合成导致了失败的第一个细胞动力学和随后的分裂失败.
- 抑制辅助精子似乎与胚胎核介导的中枢复制的抑制有关.
结论:
- 胚胎核在调节多精子新蛋中的辅助精子命运方面发挥着至关重要的作用.
- 胚胎细胞核对中心复制的抑制是防止辅助精子参与裂变的关键机制.
- 适当的细胞周期进展和DNA合成对于成功的细胞动力学和胚胎发育至关重要.
相关概念视频
01:57Meiosis II
Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each containing...
01:41Spermatogenesis
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
01:38Fertilization
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
01:34What is Meiosis?
Meiosis is the process by which diploid cells divide to produce haploid daughter cells. In humans, each diploid cell contains 46 chromosomes, half from the mother and half from the father. Following meiosis, the resulting haploid eggs or sperm only contain 23 chromosomes; however, each of these chromosomes contains a unique combination of parental information that results from the meiotic process of crossing over.
Although meiosis shares similarities with mitosis—both rely on microtubules to...
Although meiosis shares similarities with mitosis—both rely on microtubules to...