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这种突变首先会在怀孕8天的三/三小鼠胚胎中引起原始生殖细胞缺乏
Takayuki Sakurai1,2, Taisen Iguchi1, Kazuo Moriwaki3
1Graduate School of Integrated Science, Yokohama City University, Yokohama 236, Japan.
Development, growth & differentiation
|June 7, 2023
概括
这种突变会影响小鼠胚胎中原始生殖细胞 (PGC) 数量,大约发生性交后8.0天. 这表明ter基因对PGC的增殖和生存至关重要.
科学领域:
- 发育生物学是发展生物学.
- 遗传学 遗传学 是一个
- 生殖生物学 生殖生物学
背景情况:
- 已知ter (teratoma) 突变会导致原始生殖细胞 (PGC) 缺乏.
- 之前的研究观察到,在较晚的发育阶段 (性交后9.5-12.5天) 的特定小鼠菌株中存在这种缺陷.
研究的目的:
- 准确研究突变对PGC发育的影响.
- 为了确定由突变引起的PGC缺乏的确切时间和性质.
主要方法:
- 在先天C57BL/6J-ter小鼠胚胎 (性交后7.5-12.5天) 中分析PGC数量和分布.
- 使用聚合酶链反应 (PCR) 进行胚胎的基因定型,用于 Grl-1 locus 微卫星 DNA 多态化.
- 检查胚胎的完整序列切口.
主要成果:
- 在ter/ter和正常胚胎中,在性交后7.5天后,PGC数量与正常胚胎相似.
- 在ter/ter胚胎中的PGC数量并没有从共生后8.0-12.5天增加,与正常 littermates不同.
- PGC向生殖器脊的迁移没有受到影响;在异构合体 (+/ter) 和野生类型 (+/+) 胚胎之间没有观察到PGC数量差异.
结论:
- 这种突变不会影响PGC在性交后7.5天的最初出现.
- PGC缺乏症首先发生在性交后8.0天左右,与迁移和扩散的开始相吻合.
- 第三基因产物可能对PGC扩散或生存机制至关重要.
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