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Updated: Jul 17, 2026

Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach
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颜色细胞在子胚胎神经视网膜培养中的差异化

Y Itoh1,2, T S Okada1, H Ide2

  • 1Institute for Biophysics, Faculty of Science, University of Kyoto, Sakyo-ku, Kyoto 606, Japan.

Development, growth & differentiation
|June 7, 2023
PubMed
概括

来自胚胎的神经视网膜细胞可以在培养中分化为色素细胞. 这一过程通过细胞计数和铁酶活性得到证实,表明色素细胞来源于神经视网膜,而不是污染物.

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相关概念视频

Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...