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Updated: Jul 27, 2026

Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach
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Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach

Published on: November 2, 2015

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在胚胎小神经视网膜的培养中对其他差异化途径的实验性操纵

R M Clayton1, D DE Pomerai1, D J Pritchard1

  • 1Institute of Animal Genetics, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh, U. K.

Development, growth & differentiation
|June 7, 2023
PubMed
概括

胚胎神经视网膜细胞可以在培养中转化为色素细胞或透镜纤维. 培养条件,如细胞密度和中型,影响了细胞命运的决定,影响了发育生物学研究.

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Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs06:58

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

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相关概念视频

Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...