相关实验视频
Updated: Aug 9, 2026
10:19
Derivation of Mouse Trophoblast Stem Cells from Blastocysts
Published on: June 8, 2010
18.3K
从小鼠胚芽细胞中建立胚胎衍生的干细胞 (ES) 细胞系:Feeder细胞层的影响
Hirofumi Suemori1, Norio Nakatsuji1
1Division of Developmental Biology, Meiji Institute of Health Science, 540 Naruda, Odawara, 250 Japan.
Development, growth & differentiation
|June 7, 2023
概括
与STO细胞相比,初级纤维细胞是建立和维持小鼠胚胎干细胞 (ESC) 线的高级料层. 在纤维细胞上培养的ESC保持双胞胎细胞类型,并表现出更广泛的分化潜力.
科学领域:
- 发展生物学 发展生物学
- 干细胞生物学 干细胞生物学
- 遗传学 遗传学 是一个
背景情况:
- 胚胎干细胞 (ESC) 线是从胚芽细胞的内部细胞质中衍生出来的.
- 料电池层对于ESC隔离和维护至关重要.
- 不同的料电池类型可能会影响ESC特性.
研究的目的:
- 为了比较STO细胞与初级胚胎纤维细胞作为小鼠ESC建立和维护的料层的有效性.
- 评估料细胞类型对ESC差异化潜力和型稳定性的影响.
主要方法:
- 从129或C57BL/6菌株中建立小鼠ESC线,使用STO养细胞或初级胚胎纤维细胞.
- 在体内差异化试验:将ESC注射到同源性小鼠中,以形成固体瘤.
- 已建立的ESC线路的型分析 (G-banding).
主要成果:
- 在STO细胞上培养的ESCs产生了近四平状核细胞型,并分化为各种组织.
- 在初级纤维细胞上培养的ESCs表现出具有正常染色体组的主要双倍化型,并显示出更广泛的分化到不同的组织中.
- 初级纤维细胞支持更好的ESC建立和维护.
结论:
- 初级胚胎纤维细胞是比STO细胞更有效的养层,用于建立和维持小鼠ESC线.
- 料细胞类型显著影响ESC型稳定性和差异化潜力.
- 双胞胎型和广泛的差异化能力是强壮的ESC线条的理想特征.
相关概念视频
00:58Embryonic Stem Cells
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
00:57Embryonic Stem Cells
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
01:14Maintenance of the ES Cell State
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...