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由于1-METHYLADENINE引起的星鱼体的刚性显著下降.

Shin-Ichi Nemoto1, Mitsuki Yoneda2, Isao Uemura3

  • 1Tateyama Marine Laboratory, Ochanomizu University, Tateyama, Chiba 294-03.

Development, growth & differentiation
|June 7, 2023
PubMed
概括

未成熟的海星卵细胞在由1-甲基氨酸 (1-MeAde) 诱导的成熟过程中变得不那么刚硬. 这种硬度的变化表明早期的细胞质事件之前的生殖囊泡分解.

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PERIODIC CHANGES IN THE CONTENT OF PROTEIN-BOUND SULFHYDRYL GROUPS AND TENSION AT THE SURFACE OF STARFISH OOCYTES IN CORRELATION WITH THE MEIOTIC DIVISION CYCLE.

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Ultrastructural Basis of the Tension Increase in Sea-Urchin Eggs Prior to Cytokinesis: (microfilament/sea-urchin egg/tension).

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MORPHOLOGICAL OBSERVATIONS ON THE SPERMATOZOA OF ECHINOTHURID SEA URCHINS.

Development, growth & differentiation·2023

科学领域:

  • 发展生物学 发展生物学
  • 细胞力学 细胞力学
  • 生殖生物学 生殖生物学

背景情况:

  • 不成熟的海星卵细胞表现出极端的刚性.
  • 成熟涉及显著的细胞变化.

研究的目的:

  • 为了研究成熟期间卵细胞刚性的变化.
  • 识别成熟过程的早期指标.

主要方法:

  • 使用压缩方法测量卵细胞刚度.
  • 使用1-甲基氨酸 (1-MeAde) 和dithiothreitol诱导成熟.
  • 使用细胞素B来评估硬度的可逆性和核化.

主要成果:

  • 卵细胞刚度在1-MeAde治疗后5-9分钟内显著下降,先于胚芽囊泡的分解.
  • 迪三醇诱导了类似的硬度变化.
  • 细胞素B可逆性地降低了硬度,而不影响成熟,并使核分裂成为可能.
  • 非核酸碎片保留了刚性,这也随着1-MeAde处理而减少.

结论:

  • 卵细胞硬度作为早期成熟事件的定量标记.

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  • 影响性的细胞质变化对于生殖囊泡的分解至关重要.
  • 影响细胞骨部件的机制在卵细胞成熟中发挥作用.