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在一个Anuran幼虫的胃: (Lepidobatrachus laevis/pepsinogen)
Edward J Carroll1, A Milinda Seneviratne1, Rodolfo Ruibal1
1Department of Biology, University of California, Riverside, California 92521 U.S.A.
Development, growth & differentiation
|June 7, 2023
概括
这项研究跟踪了青 (Lepidobatrachus laevis) 幼虫的胃发育,发现素的生产开始较早. 素的活性和水平随着幼虫的变形而显著增加.
科学领域:
- 发展生物学 发展生物学
- 比较生理学比较生理学
- 胃肠病学 胃肠病学
背景情况:
- 义务食肉青Lepidobatrachus laevis经历了显著的幼虫发育.
- 了解消化酶本体发生对于幼虫的生存和生长至关重要.
研究的目的:
- 调查Lepidobatrachus laevis幼虫素生产和活动的发育时间表.
- 描述幼虫发育过程中存在的pepsinogen同酶.
主要方法:
- 使用抗猪素抗体对产生素的细胞进行免疫组合化学鉴定.
- 在低pH下,在孤立的幼虫胃中对活动的微试验.
- 用类分析试验对类原体进行电泳分析.
主要成果:
- 早在第24阶段 (眼球发育) 发现了素的产生,这与养能力相吻合.
- 每个胃的胃部活动总量增加了5,400倍,从幼虫早期到变形阶段的特定活动增加了345倍.
- 电泳检测显示了24阶段的一个主要的pepsinogen,两个额外的异酶在后期的发育中出现. 确定异素的分子量为34,800.
结论:
- 素存在并从Lepidobatrachus laevis幼虫的早期食阶段开始活跃.
- 在幼虫发育过程中,素发生了显著的定量和质量变化,支持过渡到变形.
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