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阿努兰白的初级蛋包:在发育过程中发生的物理化学和宏分子变化: (蛋包)

Thomas R Peavy¹, Edward J Carroll¹

¹Department of Biology University of California Riverside, California 92521.

Development, growth & differentiation

|June 7, 2023

概括

青Lepidobatrachus laevis的蛋包 (CE,VE,FE) 含有类似的主要多. 蛋白酶活动可能会在受精和化过程中改变这些包裹.

科学领域:

生殖生物学生殖生物学
发育生物学是发展生物学.
分子生物学分子生物学

背景情况:

青Lepidobatrachus laevis的体蛋包 (CE) 具有纤维状束,可以在卵管过渡后分散.
产卵后,青蛋的状外 (VE) 呈现出一个外部无形区域.
在受精后形成的受精包裹 (FE) 非常类似于VE.

研究的目的:

描述青蛋封的多组合 (CE,VE,FE和化FEh).
研究这些包裹的转化和潜在的酶处理.
为了探索Lepidobatrachus laevis和Xenopus蛋包组件之间的交叉反应.

主要方法:

手动隔离结肠膜包裹 (CE),维他林膜包裹 (VE),受精膜包裹 (FE) 和化膜包裹 (FEh).
在变质和非变质条件下,封装的溶解.
使用凝电泳和一维映射进行分析.
用激活的卵液和化酶进行化,然后进行三和三消化.
使用针对Xenopus VE的抗体进行免疫学交叉反应性测试.

主要成果:

所有分析的包裹 (CE,VE,FE,FEh) 含有9种主要多 (118.522 kD) 和812种次要多.
在化后,用卵液和化酶修改 (添加/删除) 了信封组件,模仿了形/形消化.
莱比多巴特拉库斯 (Lepidobatrachus laevis) 的CE多和VE,FE和FEh的主要成分与抗体对Xenopus VE.表现出交叉反应.

结论:

莱比多巴特拉丘斯 (Lepidobatrachus laevis) 的蛋包共享保存的多成分,有酶加工的证据.
蛋白酶很可能参与青蛋包的体内加工.
在Lepidobatrachus laevis和Xenopus的蛋包蛋白之间存在免疫学上的相似性.

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