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相关概念视频

Induced Pluripotent Stem Cells01:06

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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
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Cancer-Critical Genes I: Proto-oncogenes01:33

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Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
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Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Regulation of Expression at Multiple Steps01:23

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
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A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
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过度表达自己的感受.

Jinpiao Zhu1, Chang Chen1, Zhen Li2

  • 1Department of Anesthesiology, Brain Research Center, Department of Neurosurgery, Zhongnan Hospital, Wuhan University, Wuhan, Hubei Province, China.

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概括
此摘要是机器生成的。

慢性睡眠不足会影响认知和大脑功能. Sirt6蛋白水平下降,但恢复Sirt6可以改善认知缺陷和大脑连接,为睡眠障碍提供潜在的治疗方法.

关键词:
快速眼睛运动 (REM) 睡眠是什么?在Sirt6上,你会看到Sirt6.慢性睡眠剥夺 慢性睡眠剥夺认知障碍是一种认知障碍.功能连接性的功能连接性谷氨酸作用的神经元代谢动态的代谢动态学神经神经细胞葡萄糖代谢在前肢皮层前的皮层.突触功能是突触的功能.

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科学领域:

  • 神经科学是一个神经科学.
  • 代谢调节 代谢调节 代谢调节 代谢调节
  • 睡眠科学 睡眠科学

背景情况:

  • 睡眠对能量代谢,神经元可塑性和认知功能至关重要.
  • Sirt6是一种依赖NAD+的脱乙酶,是影响转录和酶途径的能量代谢的关键调节剂.
  • 慢性睡眠不足 (CSD) 会对大脑功能和认知产生负面影响.

研究的目的:

  • 为了研究Sirt6在CSD后大脑功能中的作用.
  • 确定Sirt6调制是否可以减轻CSD诱导的认知和神经缺陷.

主要方法:

  • 在C57BL/6J小鼠中,在前皮层 (PrL) 中进行了CSD和病毒载体介导的Sirt6过度表达.
  • 通过静止状态fMRI评估大脑功能连接 (FC),使用代谢动力学 ([1-13C]葡萄糖和[2-13C]乙酸盐) 的神经元/星细胞代谢,树突脊柱密度和突触活动 (mEPSCs,AP发射率).
  • 用全面的行为测试来评估认知功能.

主要成果:

  • CSD导致PrL中的Sirt6水平降低,认知缺陷,并在PrL和多个大脑区域之间减少了FC.
  • Sirt6过度表达逆转了CSD诱导的认知障碍,并使FC正常化.
  • 中枢神经干细胞减少了神经元中的葡萄糖和GABA合成,而Sirt6的过度表达恢复了这种情况.
  • Sirt6过度表达正常化了CSD引起的动作潜能发射速率和微型刺激后突触电流的缺陷.

结论:

  • Sirt6在缓解CSD引起的认知障碍和神经功能障碍方面发挥着至关重要的作用.
  • 通过调节与PrL相关的功能连接,神经元代谢和谷氨酸性神经传递,Sirt6可以改善认知缺陷.
  • 激活Sirt6为睡眠障碍相关的认知障碍提供了潜在的治疗策略.