胰岛素对骨形成的影响 鼠气膜骨髓骨髓介质干细胞的能力
Lingling E1, Rongjian Lu2, Ying Zheng1,3
1Institute of Stomatology and Oral Maxilla Facial Key Laboratory, First Medical Center of Chinese PLA General Hospital, Beijing, China.
Stem cells and development
|June 7, 2023
概括
胰岛素通过促进膜骨髓中介质干细胞 (ABM-MSCs) 的增殖和骨质分化,显著增强口腔骨愈合. 这种效应通过胰岛素/mTOR信号通路进行介导,暗示胰岛素.
科学领域:
- 口服再生医学是一种口服再生医学.
- 干细胞生物学 干细胞生物学
- 内分泌学 在内分泌学.
背景情况:
- 膜骨髓介质干细胞 (ABM-MSCs) 对于口腔骨修复至关重要.
- 胰岛素在改善受损的口腔骨状况方面的作用是公认的.
- 胰岛素对ABM-MSC骨质性潜力的特定影响需要澄清.
研究的目的:
- 为了研究胰岛素对大鼠ABM-MSCs的增殖和骨质分化的影响.
- 阐明潜在的分子机制,特别是胰岛素/mTOR信号通路.
- 为了评估胰岛素在促进骨形成中的有效性,在体外和体内.
主要方法:
- 在老鼠中培养ABM-MSC并用不同度的胰岛素治疗它们.
- 评估细胞增殖,性酸酶 (ALP) 活性,I型原 (COL-1) 合成和骨素 (OCN) 表达.
- 分析胰岛素受体 (IR) 和哺乳动物目标的拉巴胺素 (mTOR) 信号通路激活.
- 在体外和体内对β-三酸 (β-TCP) 支架进行ABM-MSC骨质生成的评估.
主要成果:
- 胰岛素以剂量依赖的方式促进了ABM-MSC的扩散,其中10−6M显示出最显著的效果.
- 胰岛素显著增强了COL-1合成,ALP活性,OCN表达和矿物化矩阵形成.
- 胰岛素治疗激活了胰岛素/mTOR信号通路,这对于骨质分化至关重要.
- 在体内研究表明,在接受胰岛素治疗的ABM-MSC患者中,骨形成和血管新生增强.
结论:
- 胰岛素在体外直接促进ABM-MSC的增殖和骨质分化.
- 胰岛素在体内增强了ABM-MSC的骨质生成和血管生成,特别是在与β-TCP结合时.
- 胰岛素/mTOR信号通路调解胰岛素对口腔骨再生的ABM-MSCs的合成效应.
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