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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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相关实验视频

Updated: Jul 27, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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高通量测序揭示了N个

Furong Wu1, Shengyu Zhang1, Chang Fan2,3

  • 1Department of Pharmacy, Division of Life Sciences and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, Anhui, China.

Current gene therapy
|June 7, 2023
PubMed
概括

长非编码RNA (lncRNAs) 中的N6-甲基氨酸 (m6A) 修饰模式与肝纤维化 (LF) 有关. 这项研究确定了特定的m6A修饰的lncRNA,揭示了它们独特的甲基化模式和在LF进展中的潜在作用.

关键词:
这是一种N6-甲基氨酸.高通量测序的高通量测序肝脏纤维化 肝脏纤维化在ncRNA中,我们可以meRIP-qPCRR是什么意思监管网络监管网络监管网络监管网络监管

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科学领域:

  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 在RNA生物学,RNA生物学.

背景情况:

  • N6-甲基氨酸 (m6A) 是一种普遍存在的RNA修饰.
  • 长非编码RNAs (lncRNAs) 是关键的调节分子.
  • m6A和lncRNA都与肝纤维化 (LF) 有关.

研究的目的:

  • 研究m6A修饰的lncRNAs在肝纤维化进展中的作用.
  • 为了确定特定的m6A甲基化lncRNAs参与LF.
  • 为了阐明LF中的lncRNAs的m6A甲基化模式.

主要方法:

  • 肝脏组织的组织病理学检查 (HE和马森染色).
  • 用m6A修饰的RNA免疫沉降测序 (m6A-seq) 来分析lncRNA甲基化.
  • RNA测序 (RNA-seq) 用于评估 lncRNA 表达水平.
  • MeRIP-qPCR和RT-qPCR用于验证目标 lncRNAs.

主要成果:

  • 在LF组织中,在313个lncRNA中确定了415个m6A峰值,在84个lncRNA中发现了98个显著峰值.
  • 发现了三种lncRNAs (H19,Gm16023,Gm17586) 在m6A甲基化和表达水平上都有显著变化.
  • 观察到m6A水平的明显变化 (H19和Gm17586的增加,Gm16023的减少) 和RNA表达的减少.
  • 构建了一个lncRNA-miRNA-mRNA调节网络以推断功能关系.

结论:

  • 证明了一种独特的m6A甲基化场景用于肝纤维化中的lncRNAs.
  • 提供了证据表明,lncRNAs的m6A修饰与LF的发生和发展有关.
  • 突出了特定的m6A修饰的lncRNAs在LF病变发生中的潜在调节作用.