相关实验视频

Updated: Jul 27, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

超越淘汰赛:微调调节植物基因表达的调节,使用基于CRISPR-Cas的促销器编辑

Xu Tang^1,2, Yong Zhang²

¹Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, School of Life Sciences, Southwest University, Chongqing, 400715, China.

The New phytologist

|June 7, 2023

概括

由于复杂的DNA结构,CRISPR-Cas9在植物中的基因组编辑面临着促进器编辑的挑战. 需要新的工具和策略来精确控制基因表达.

科学领域:

植物分子生物学植物分子生物学
基因组编辑技术的技术.
基因调节基因调节

背景情况:

克里斯普尔-卡斯技术正在快速推进植物的基因组编辑.
编辑植物促进体的cis调节性等位基因为控制基因表达提供了一个有前途的途径.
虽然CRISPR-Cas9功能强大,但它在编辑非编码促进器区域方面存在局限性.

研究的目的:

突出使用CRISPR-Cas系统编辑植物促进剂所面临的挑战.
讨论改善工具和策略的必要性,以实现高效的促销者编辑.
通过非静音编辑事件来实现目标基因表达的精确调节.

主要方法:

审查当前的CRISPR-Cas9限制在编辑植物促进剂.
对主办方特定挑战的分析:A-T丰富性,重复序列,监管区域的识别.
讨论DNA结构,表观遗传修饰和促进者编辑中的可访问性问题.

主要成果:

由于其独特的特性,CRISPR-Cas9在编辑植物促进物方面面临重大障碍.
现有的方法在效率,多样性和实现精确,非静音编辑方面扎.
缺乏专门的工具阻碍了促进器编辑用于基因表达控制的全部潜力.

结论:

对于植物促进剂研究,迫切需要有效和可行的编辑工具.
解决主办方特定的挑战对于提高编辑效率和多样性至关重要.
开发先进的策略是必要的通过促进器编辑精确的目标基因表达调节.

关键词:

这就是CRISPR-Cas.这是一个 cis-regulatory 元素.功能性基因组学功能性基因组学调节基因表达调节基因表达分子繁殖分子繁殖没有编码的序列.植物基因组编辑植物基因组编辑发起人编辑编辑

更多相关视频

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

相关概念视频

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...