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17 在使用DMEK制剂后的细胞活力

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科学领域:

  • 眼科医生 眼科 眼科
  • 移植生物学 移植生物学
  • 角膜外科手术 角膜外科手术

背景情况:

  • 德塞梅特膜内皮皮质角膜整形术 (DMEK) 是一种用于角膜失明的手术程序.
  • 移植的准备和储存是影响结果的关键步骤.
  • 内皮细胞密度 (ECD) 和活力是DMEK移植成功的关键决定因素.

研究的目的:

  • 评估移植准备和器官培养储存对ECD和DMEK移植的生存能力的影响.
  • 确定这些过程是否会在移植前影响内皮细胞的健康.

主要方法:

  • 准备了DMEK移植 (n=27) 并对细胞活力 (Calcein-AM染色) 和ECD进行了评估.
  • 评估包括光显微镜 (LM ECD) 和素-ECD.
  • 移植品在准备后直接评估,并在储存3-7天后进行评估.

主要成果:

  • LM评估显示,在制备后立即出现一个完整的内皮单层.
  • 对于最初分配的5个移植,calcein-ECD比LM ECD低18%.
  • 配对植入物显示出最小的ECD中位数下降 (准备后1%,储存后2%).
  • 活性细胞覆盖率仍然很高 (88%在制备后,92%在储存后).

结论:

  • 移植的准备和储存通常保持内皮细胞的活力.
  • 一些移植可能会出现早期的内皮细胞损伤,但储存会导致额外的变化很少.
  • 在眼睛银行实施移植前的EDD评估可能会改善DMEK的结果.