[对Cinnamomum camphora中关键烯合成酶的化学形式的系统识别]
1State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
概括
研究人员在Cinnamomum camphora中确定了 (+) - 波伦醇生物合成的关键酶基因. 这一发现促进了对其化学多样性的理解,并使生物工程能够生产高产玻利.
科学领域:
- 植物生物化学 植物生物化学
- 分子生物学分子生物学
- 化学生态化学生态学
背景情况:
- (Cinnamomum camphora) 是一种有价值的中国树种,其叶子具有多样化的挥发性油成分.
- 存在五种化学类型,包括醇,坎佛,林纳醇,金醇和尼罗利多尔类型,受合成酶 (TPS) 酶的影响.
- 对于经济上重要的 (+) - 波伦醇的生物合成途径仍然未被阐明.
研究的目的:
- 为了识别参与Cinnamomum camphora (+) - 波伦醇生物合成的关键酶基因.
- 描述九个克隆的合成酶基因 (CcTPS1-CcTPS9) 的酶活性.
- 为生物工程高产玻利油品种奠定基础.
主要方法:
- 对四种C. camphora化学型的转录组分析,以确定九种合成酶基因 (CcTPS1-CcTPS9).
- 在大肠杆菌中克隆和重组蛋白质表达.
- 在实验室中使用格拉尼尔酸盐 (GPP) 和法纳西尔酸盐 (FPP) 作为基质的酶分析.
主要成果:
- CcTPS1 和 CcTPS9 催化了 GPP 产生玻里酸盐,在水解后产生 (+) - 玻里 (分别为0.4% 和 89.3%).
- CcTPS3和CcTPS6从GPP中产生了林纳醇;CcTPS6也从FPP中产生了尼罗利多尔.
- 在CcTPS8中,从GPP中生产了1,8-cineole (30.71%). 总的来说,九个TPS酶产生了9个单二烯和6个六二烯.
结论:
- 这项研究确定了关键酶基因 (CcTPS1和CcTPS9),它们负责C. camphora.中的 (+) - 波伦醇生物合成.
- 这为了解化学型形成机制提供了基础.
- 允许未来的生物工程努力,以提高玻利生产.
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