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[基于BDNF/TrkB/CREB通路的Shenling Kaixin颗粒的抗抑郁机制]

Yan Xu¹, Dong-Guang Liu¹, Ting-Bo Ning¹

¹New Drug Pharmacological Center of Lunan Pharmaceutical Group Corporation Linyi 276006, China State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine Linyi 276006, China.

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

|June 7, 2023

概括

沈灵凯辛颗粒 (SLKX) 通过减少老鼠的压力诱导的炎症和氧化损伤,显示出抗抑郁作用. SLKX治疗对行为和神经化学标记有积极的影响,这表明它在海马中起着保护作用.

科学领域:

神经科学是一个神经科学.
药理学药理学是指药理学的学科.
传统中国医药传统中国医药

背景情况:

慢性不可预测的轻度压力 (CUMS) 是一种常见的模式,用于诱导动物的类似抑郁的行为.
了解抑郁症背后的分子机制对于开发有效治疗方法至关重要.
像Shenling Kaixin Granules (SLKX) 这样的草药因其潜在的抗抑郁药物特性而受到探索.

研究的目的:

在慢性不可预测的轻度压力 (CUMS) 的老鼠模型中阐明Shenling Kaixin颗粒 (SLKX) 的抗抑郁机制.
评估SLKX对与抑郁症相关的行为,生化和分子变化的影响.

主要方法:

建立一个CUMS老鼠模型来模拟抑郁.
行为评估包括糖类偏好,开放场地,高架十字迷宫和强迫游泳测试.
对血清炎症性细胞因子 (IL-1β,TNF-α),神经递质 (5-HT) 和抗氧化酶活性 (SOD,CAT) 的生物化学分析.
通过西斑和HE染色,对海马蛋白质 (NGF,BDNF,TrkB,CREB,Nrf2,HO-1,Bcl-2,Bax,caspase-3) 的分子分析.

主要成果:

CUMS大鼠表现出类似抑郁症的行为,血清IL-1β和TNF-α增加,卡斯巴酶-3表达升高,海马BDNF,5-HT,SOD和CAT活动减少.
SLKX治疗显著改善了行为结果,减少了炎症标志物和caspase-3表达,并增强了海马中的神经营养因子和抗氧化能力.
SLKX上调了NGF,BDNF,p-TrkB/TrkB,p-CREB/CREB,Nrf2,HO-1和Bcl-2/Bax的表达,同时降低了Bax和caspase-3的表达,表明神经保护和抗亡作用.

结论:

申林凯辛颗粒 (SLKX) 通过调节BDNF/TrkB/CREB通路并激活Nrf2核转位,产生抗抑郁作用.
SLKX减轻海马体的氧化应激和炎症,抑制细胞亡,促进神经保护,从而减轻类似抑郁症的症状.
这些发现支持SLKX作为潜在的抑郁症治疗剂,提供了一种涉及神经营养和抗氧化途径的新型作用机制.

关键词:

通过BDNF/TrkB/CREB通路进行.Nrf2/HO-1通道中的一个.沈凌凯信颗粒沈凌凯信颗粒卡斯巴-3 途径慢性不可预测的轻度压力 (CUMS)抑郁抑郁症抑郁症抑郁症是一种

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