乙酸和c-di-GMP的相遇调节了BldD活动,用于开发和生产抗生素
Yu Fu1, Yu-Qi Dong1, Jin-Long Shen1
1Laboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Nucleic acids research
|June 7, 2023
概括
环境应激触发乙酸 (AcP) 调节细菌的发展和抗生素的生产,通过修改BldD蛋白质,整合c-di-GMP信号.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 动态细菌表现出与抗生素生产相关的复杂发育过渡,由c-di-GMP和BldD抑制剂调节.
- 在Actinobacteria中管理这些过程的上游调节器和全球信号网络在很大程度上是未知的.
研究的目的:
- 阐明上游因素和信号网络控制发展过渡和抗生素生产在Actinobacteria.
- 研究乙酸 (AcP) 在调节BldD活动中的作用及其与c-di-GMP信号的整合.
主要方法:
- 研究了环境应激对Saccharopolyspora erythraea中乙烯酸 (AcP) 水平的影响.
- 使用生物化学和遗传方法分析了AcP,c-di-GMP和BldD抑制剂之间的相互作用.
- 研究了BldD乙化在K11对DNA结合,二元化和下游信号传导的影响.
主要成果:
- 在应激下酸 (AcP) 的积累通过K11的乙化调节了BldD活性,导致BldD二元从DNA中解离.
- 由AcP诱导的BldD乙化破坏了c-di-GMP信号传导,影响了细菌的发育和抗生素的产生.
- 一个BldDK11R突变,耐乙化,增强了BldD对抗生素生产的积极影响.
结论:
- 乙酸 (AcP) 在通过共价改性来调节BldD活性方面发挥着新的作用,与c-di-GMP信号集成,以控制发展和抗生素生产,以应对环境压力.
- 这种涉及AcP依赖BldD乙化的调节机制,代表着一种独特的共价变异模式,并为细菌应激反应提供了新的视角.
- 已识别的调节网络可能在Actinobacteria中保存,这对理解和操纵抗生素生物合成有重大影响.
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