全球传播来自人类肠道微生物群的广域宿主等离子体
Lili Yang1, Guoqin Mai1, Zheng Hu1
1CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Nucleic acids research
|June 7, 2023
概括
人体肠道中的广域宿主等离子体在很大程度上是未知的. 这项研究确定了许多等离子体,揭示了其中一些可以在全球传播,促进横向基因转移并影响人类健康.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 流行病学 流行病学
背景情况:
- 广泛宿主范围 (BHR) 质体对于细菌的水平基因转移 (HGT) 是至关重要的.
- 在人类肠道微生物群中,BHR等离子体的多样性和发病率在很大程度上仍未被描述.
研究的目的:
- 在人类肠道细菌基因组中识别和表征等离子体,特别是BHR等离子体.
- 调查这些等离子体的流行,宿主范围和全球分布.
主要方法:
- 分析来自不同人群的肠道细菌分离物的基因组草案.
- 基于完整性和复制体类型的等离子体类集群 (PLC) 的识别和分类.
- 哈普洛型分析以推断广泛传播的等离子体的进化轨迹.
主要成果:
- 确定了5372个类似等离子体的集群 (PLC),其中820个 (comPLC) 符合完整性标准.
- 只有18.9%的comPLC属于已知的复制体类型,这表明了新的等离子体多样性.
- 175个comPLC表现出广泛的宿主范围,其中71个在多个人类群体中发现,13个在至少一个人群中高度流行.
结论:
- 建立了大量的人类肠道等离子体序列的集合.
- 一个BHR等离子体的子集表明了全球传播潜力,促进了广泛的HGT基因,如抗生素耐药性.
- 这些发现强调了肠道等离子体对全球人类健康和疾病传播的重大影响.
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