相关实验视频

Updated: Jul 27, 2025

Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification
08:46

Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification

Published on: May 18, 2020

6.0K

使用TLC-CLIPIP进行RNA-蛋白相互作用的高效和敏感的分析

Christina Ernst1, Julien Duc1, Didier Trono1

  • 1Global Health Institute, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.

Nucleic acids research
|June 7, 2023
PubMed
概括

我们开发了一种新的方法,cDNA (TLC) -CLIP的尾巴和结合,用于分析RNA-蛋白相互作用. 这种技术显著提高了灵敏度,允许从最小的细胞样本进行分析,并加强基因调节研究.

更多相关视频

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

5.1K
iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution10:45

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

Published on: April 30, 2011

58.5K

相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K