基本纤维细胞生长因子诱导由由牛肉体白色体††衍生的纤维细胞的增殖和原生产
Corrine F Monaco1,2, Michele R Plewes1,3, Emilia Przygrodzka1
1Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, NE, USA.
Biology of reproduction
|June 7, 2023
概括
纤维细胞生长因子2 (FGF2) 在黄体回归过程中激活黄体纤维细胞. 这种FGF2信号促进纤维细胞的增殖和原蛋白的产生,影响质微环境.
科学领域:
- 生殖生物学 生殖生物学
- 细胞和分子生物学是细胞和分子生物学.
- 细胞外矩阵重塑 细胞外矩阵重塑
背景情况:
- 黄体回归涉及到快速的矩阵重塑.
- 纤维细胞在回归的黄色体中的作用基本上是未知的.
- 转录基因转移包括在质回归过程中减少VEGFA和增加FGF2.
研究的目的:
- 调查FGF2在黄体回归过程中激活黄体纤维细胞中的作用.
- 了解牛状纤维细胞对FGF2.2的下游信号传递,原蛋白生产和增殖反应.
主要方法:
- 诱导色素回归的转录学分析.
- 用FGF2.2治疗牛肉状纤维细胞.
- 下游信号通路 (ERK,AKT,STAT1) 的测量.
- 评估1型原蛋白的产生和纤维细胞的增殖.
主要成果:
- FGF2迅速诱导ERK,AKT和STAT1信号通路的酸化.
- FGF2 显示了对原蛋白生产的度依赖作用.
- FGF2充当了线粒原体,促进纤维细胞增殖,这取决于AKT和STAT1的信号传递.
- 纤维细胞激活和纤维化标志物 (FAP,SERPINE1,SPP1) 在色回归过程中升高.
结论:
- 黄体纤维细胞对在牛体黄体回归过程中释放的因素做出反应.
- FGF2在激活白质纤维细胞,影响增殖和细胞外基质产生方面发挥着重要作用.
- 这些发现为纤维细胞对回归的黄体微环境的贡献提供了洞察力.
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