对胸前大动脉动脉瘤和剖析中与衰老相关的基因进行生物信息学分析
Hong Wan1, Danlingyi Liu1, Bingqing Liu1
1School of Medical Technology, Beihua University, Jilin, China.
Frontiers in cardiovascular medicine
|June 7, 2023
概括
衰老显著影响胸前大动脉动脉瘤和解剖 (TAAD). 这项研究确定了包括MYC和ESR1在内的关键基因,具有与衰老相关的TAAD的诊断潜力,突出了HIF-1信号通路的作用.
科学领域:
- 心血管生物学 心血管生物学
- 衰老研究研究 衰老研究
- 基因组学就是基因组学.
背景情况:
- 胸前动脉动脉瘤和解剖 (TAAD) 是一种危及生命的心血管疾病.
- 衰老是TAAD的主要风险因素,需要研究潜在的分子机制.
- 了解衰老和TAAD之间的相互作用对于开发有效的诊断和治疗策略至关重要.
研究的目的:
- 探索衰老过程与TAAD发展之间的复杂关系.
- 为了确定关键的基因和分子途径,涉及到与衰老相关的TAAD.
- 调查TAAD发现基因的诊断潜力.
主要方法:
- 利用了人类衰老基因数据,并下载了TAAD的各种基因表达综合 (GEO) 数据集.
- 在衰老和TAAD之间选差异表达基因 (DEGs) 和差异共同表达的基因.
- 雇佣基因本体学 (GO),基因和基因组的京都百科全书 (KEGG),基因组丰富分析 (GSEA) 和蛋白质与蛋白质相互作用 (PPI) 网络分析.
- 使用Cytoscape软件识别了枢纽基因,并通过单细胞RNA测序验证了它们的表达.
- 使用接收器操作特征 (ROC) 曲线评估诊断潜力.
主要成果:
- 在衰老和TAAD之间确定了70个差异性共同表达的基因.
- 富化分析揭示了DNA代谢,细胞衰老和HIF-1信号通路中的重要作用.
- 确定了五个枢纽基因 (MYC,IL6,HIF1A,ESR1,PTGS2),MYC和ESR1具有特别的诊断价值.
- 单细胞测序证实了大动脉组织中枢基因的差异表达.
- 结合的ROC曲线分析表明,已识别的枢纽基因具有显著的诊断能力.
结论:
- HIF-1信号通路被认为是衰老和TAAD的关键因素.
- MYC和ESR1成为与衰老相关的TAAD的有前途的诊断生物标志物.
- 对这些基因和通路的进一步研究可能会导致TAAD管理的改善.
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