介酶体 stromal 细胞外体增强牙纤维细胞功能,促进牙纤维-牙再生
Jiajun Shi1, Kristeen Ye Wen Teo1, Shipin Zhang1
1Faculty of Dentistry, National University of Singapore, Singapore.
Biomaterials and biosystems
|June 7, 2023
概括
介酶体 stromal/干细胞 (MSC) 外体通过促进细胞迁移,增殖和分化来增强牙纸再生. 这些无细胞囊泡为纸牙修复提供了一个有希望的替代方案.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 细胞生物学 细胞生物学
背景情况:
- 间酶体 stromal/干细胞 (MSCs) 在牙髓再生方面进行了研究.
- 多细胞细胞的治疗作用主要由细胞外囊泡 (EVs) 介导,包括外体.
- 了解外体介导机制对于开发无细胞疗法至关重要.
研究的目的:
- 为了研究MSC外体在牙髓再生中的细胞和分子机制.
- 评估MSC外体在促进牙髓细胞功能和组织形成方面的疗效.
主要方法:
- 牙纸细胞 (DPC) 培养物用MSC外体进行了处理.
- 评估了细胞过程 (迁移,增殖,分化).
- 在体内研究中使用了老鼠纸缺陷模型和在小鼠体内植入的内牙治疗的人类前牙.
主要成果:
- MSC外体显著增加了DPC迁移,增殖和牙产生差异化.
- 鉴定出外体CD73介导的AKT和ERK信号的腺受体激活是关键机制.
- 在体内,MSC外基因组促进了牙状组织和桥梁形成,与MTA治疗相似.
结论:
- 通过对DPC功能的多方面影响,MSC外体促进牙髓再生.
- MSC外体体代表了一种可行的无细胞治疗替代品,用于纤维-牙再生.
- 这项研究为MSC外体在牙科中的临床应用提供了基础.
相关概念视频
01:19Mesenchymal Stem Cells
4.8K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.8K
01:21Stem Cell Therapy for Tissue Regeneration
4.1K
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
4.1K