构成性和昆虫诱导的转录组的虫耐药和敏感的西卡杉
Justin G A Whitehill1,2, Macaire M S Yuen1, Jörg Bohlmann1,3,4
1Michael Smith Laboratories University of British Columbia Vancouver BC Canada.
Plant-environment interactions (Hoboken, N.J.)
|June 7, 2023
概括
西特卡杉的虫耐药性涉及不同的构成性基因表达模式. 耐药树木表现出防御型的转录组,而易感树木表现出以生长为重点的转录组,暗示了微生物组的作用.
科学领域:
- 植物与昆虫的相互作用
- 森林昆虫学 森林昆虫学
- 分子生态学分子生态学
背景情况:
- 虫 (Pissodes strobi) 是北美主要的森林害虫,影响着树和松树的再生.
- 幼虫在年轻树木上食会导致显著的生长减少和死亡率.
- 了解宿主抵抗机制对于森林管理至关重要.
研究的目的:
- 描述抗虫 (R) 和易受虫 (S) 之间的构成性和诱导性转录基因差异 锡特卡杉 (Picea sitchensis).
- 识别与杉虫耐药性和易感性相关的基因表达模式.
- 探索微生物组在这种相互作用中的潜在作用.
主要方法:
- 用RNA测序 (RNA-seq) 来分析西特卡杉的转录组.
- 进行差异表达 (DE) 分析以确定显著的基因表达变化.
- 开发了一个具有2x3因数设计的统计模型,以区分昆虫诱导的反应与机械损伤效应.
主要成果:
- 确定了两个主要的转录组对比:R和S树之间的构成性差异表达基因,以及虫诱导的S树中的DE基因.
- 耐药树木表现出适应防御的构成性转录组,而敏感树木则表现出与生长相关的转录组的丰富.
- 菌转录被始终检测和差异地表达,表明微生物组在宿主-害虫相互作用中的潜在作用.
结论:
- 西特卡杉对杉虫的抗性与独特的构成性转录基因特征有关,有利于抗性树木的防御.
- 易受攻击的树木表现出以生长为导向的构成性转录组,这可能使它们更容易受到虫的攻击.
- 转录的一致检测和差异表达表明微生物群在这种针叶植物-昆虫相互作用中的作用很重要,但尚未得到充分研究.
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