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在暴露于西斯后,慢性炎症和损伤中的STAT1/HMGB1/NF-κB通路

Ying Fu¹, Yu Xiang¹, Ying Wang¹

¹Department of Nephrology, Hunan Key Laboratory of Kidney Disease and Blood Purification, The Second Xiangya Hospital of Central South University, Changsha 410011, China.

|June 7, 2023

概括

重复低剂量西斯 (RLDC) 通过激活STAT1/HMGB1/NF-κB通路,导致脏损伤. 用HMGB1抗体阻断这种通路可能会保护脏免受慢性思思甲毒性影响.

科学领域:

腎臟病學 (nephrology) 是一種醫學.
在瘤学瘤学.
分子生物学分子生物学

背景情况:

西斯普拉丁化疗可能会导致损伤 (毒性).
重复低剂量西斯 (RLDC) 减少了急性损伤,但可能导致慢性脏问题.
需要新的治疗点来预防长期的西斯普拉丁诱导的病.

研究的目的:

为了研究在西斯普拉丁脏毒性中的STAT1/HMGB1/NF-κB信号通路.
探索HMGB1作为预防RLDC慢性损伤的治疗标.

主要方法:

使用RLDC小鼠模型和近端管状细胞进行体内和体外研究.
使用中和抗体和淘汰技术检查了HMGB1的作用.
使用siRNA和药理学抑制剂 (Fludarabine) 调查了STAT1.
分析了基因表达综合 (GEO) 数据和人类脏活检样本.

主要成果:

RLDC诱导管损伤,炎症和纤维化,并增加了HMGB1水平.
阻断HMGB1减少了NF-κB的激活,炎症,纤维化,并改善了功能.
STAT1 knockdown抑制了HMGB1的转录和积累,这意味着STAT1参与了HMGB1的激活.
在慢性病 (CKD) 患者的脏组织中,STAT1/HMGB1/NF-κB轴被上调.

结论:

该STAT1/HMGB1/NF-κB通路驱动持续性炎症和纤维化后,西斯的毒性.
针对这种途径,特别是HMGB1,提供了一个潜在的策略,用于保护脏在青化疗期间.

关键词:

这就是HMGB1的特点.在 NF-κBB 中.西斯普拉丁 (cisplatin) 是一种制剂.这是一种炎症炎症炎症炎症.脏纤维化脏纤维化

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