不同目标温度的冷却干预对热中风老鼠的影响
Changdong Wu1, Ping Wang1, Bin Wang1
1Xinjiang Emergency Center, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830001, People's Republic of China.
Journal of inflammation research
|June 7, 2023
概括
将热中风 (HS) 鼠冷却到基线核心体温 (Tc) 以上1°C,在减少细胞亡和组织损伤方面表现更好. 这种干预调节了PI3K/Akt通路,减少了亲亡蛋白.
科学领域:
- 身体生理学 身体生理学
- 细胞生物学 细胞生物学
- 毒理学 毒理学 毒理学
背景情况:
- 热中风 (HS) 是一种危及生命的疾病,其特征是严重的高温和多器官功能障碍.
- 有效的冷却策略对于减轻HS引起的损害至关重要,但最佳目标温度仍在研究中.
研究的目的:
- 在热中风的老鼠模型中确定冷却干预的最佳目标温度.
- 阐明在HS中冷却的保护作用背后的分子机制.
主要方法:
- 在Sprague-Dawley大鼠中建立热中风模型.
- 针对不同核心体温 (基线,基线-1°C,基线+1°C) 的冷却干预措施的应用.
- 评估PI3K/Akt信号通路中的组织病理变化,细胞亡和蛋白质表达.
主要成果:
- 热中风诱导了肺部,肝脏和脏组织的显著基因病理损伤和亡.
- 冷却干预部分缓解了HS引起的损害.
- 降温到基线核心体温+1°C显示出更好的亡减缓和肺组织中显著降低Bax表达的趋势.
- HS增加了p-Akt,Caspase-3和Bax,同时降低了Bcl-2;冷却扭转了这些变化.
结论:
- 冷却干预可以通过调节PI3K/Akt路径来缓解热中风引起的器官损伤.
- 准基线+1°C的核心体温可能会带来更大的益处,可能与减少巴克斯表达有关.
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