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相关概念视频

Genomics02:02

Genomics

36.5K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
36.5K
Sanger Sequencing01:57

Sanger Sequencing

755.2K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
755.2K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

18.9K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.9K
Next-generation Sequencing03:00

Next-generation Sequencing

91.7K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
91.7K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.3K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.3K
RNA-seq03:21

RNA-seq

10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K

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相关实验视频

Updated: Jul 27, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

22.9K

完整的基因组序列"的"

Yalu Li1, Xiao-Hua Yan2, Yanzhi Liu1

  • 1Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.

Microbiology resource announcements
|June 7, 2023
PubMed
概括

研究人员介绍了"候选植物青叶"的完整基因组序列 TB2022. 这种植物质与中国的甜小叶病有关.

科学领域:

  • 植物病理学 植物病理学
  • 细菌学 细菌学是一门学科.
  • 基因组学就是基因组学.

背景情况:

  • "Candidatus Phytoplasma aurantifolia"是一种具有经济意义的植物病原体.
  • 甜的小叶病在受影响地区造成了大量的作物损失.
  • 植物质体基因组对于理解它们的生物学和开发控制策略至关重要.

研究的目的:

  • 为了呈现"候选植物青叶"的完整基因组序列TB2022.
  • 为研究病原体的毒性和宿主相互作用提供基因组资源.
  • 帮助开发甜小叶病的诊断和管理策略.

主要方法:

  • "Candidatus Phytoplasma aurantifolia"的全基因组测序 TB2022. 这是一个非常好的方法.
  • 对生成的基因组序列的生物信息分析.
  • 一个670,073-bp圆形染色体的组合.

主要成果:

  • 成功组装了"Candidatus Phytoplasma aurantifolia" TB2022 的完整基因组序列.
  • 基因组包括一个由670,073个基因对组成的单个圆形染色体.
  • 这些基因组数据为进一步研究病原体提供了基础.

更多相关视频

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

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Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

8.7K

相关实验视频

Last Updated: Jul 27, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

22.9K
Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.1K
Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

8.7K

结论:

  • 完整基因组序列的可用性促进了对"Candidatus Phytoplasma aurantifolia"的深入分析.
  • 这一资源将有助于我们更好地了解植物等离子体与植物的相互作用和疾病机制.
  • 基因组洞察对于在甜种植中有效管理疾病至关重要.