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胚胎脊髓内化在人体干部 机体生成 胃:心脏与肠道定制以及其他方面
Zachary T Olmsted1,2, Maria Belen Paredes-Espinosa1, Janet L Paluh1
1State University of New York Polytechnic Institute, College of Nanoscale Science and Engineering, Nanobioscience, Albany, NY, USA.
Methods in molecular biology (Clifton, N.J.)
|June 7, 2023
概括
骨干偏向的人类胃类可以研究脊髓神经发生和器官发育. 这些自我组织的模型模仿了人类早期的发展,用于研究神经系统的形成.
科学领域:
- 发展生物学 发展生物学
- 干细胞生物学 干细胞生物学
- 神经科学是一个神经科学.
背景情况:
- 人类胃类动物为研究人类早期发育提供了一个模型.
- 干偏向性胃类动物集成多种细胞类型,用于复杂的发育过程.
- 现有的模型缺乏在体内观察到的自我组织.
研究的目的:
- 详细介绍了产生干偏差的人类胃类动物的协议.
- 建立研究脊髓神经发生和器官形态发生的模型.
- 为了研究肠道和心脏神经系统的形成.
主要方法:
- 诱导多能干细胞 (iPSCs) 到干细胞表型的诱导.
- 开发两种不同的协议,用于树干偏差的胃形成.
- 培养胃类动物以促进多个血统的分化和神经模式.
主要成果:
- 成功生成带有干偏差的胃类动物,具有协调的神经模式.
- 在胃类动物中表现肠道和心脏神经系统的发展.
- 建立一个ex vivo系统来研究神经集成.
结论:
- 干偏差的人类胃类素是研究早期神经发生和器官发生的强大工具.
- 这些模型总结了胚胎发育的关键方面,用于ex vivo研究.
- 这些协议允许对神经发育和集成进行可定制的调查.
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