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Updated: Jul 27, 2025

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
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DIA-MS2pep:一个没有图书馆的框架,用于从数据独立的获取数据中全面识别

Junjie Hou1, Jifeng Wang2, Fuquan Yang3,4

  • 1National Laboratory of Biomacramolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

Biophysics reports
|June 8, 2023
PubMed
概括

DIA-MS2pep提供了一种新的无库方法,用于从数据独立采集 (DIA) 质谱数据中识别. 这种方法提高了精度和灵敏度,改善了蛋白质组量化,而不依赖光谱库.

关键词:
这是DIA-MS.较大的前体质量容忍度.质谱测量质量谱测量没有光谱库的光谱图书馆.频谱去复杂化 (demultiplexing) 是一种

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Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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