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低于最小的 Gentamicin 抑制度对由临床分离物所产生的酸盐的影响

Navid Saidi¹, Fateme Davarzani¹, Zahra Yousefpour¹

¹Molecular Microbiology Research Center (MMRC), Faculty of Medicine, Shahed University, Tehran, Iran.

Advanced biomedical research

|June 8, 2023

概括

低抑制度的珍塔素影响了Pseudomonas aeruginosa的酸盐产量. 虽然大多数隔离物显示酸的增加,但有些表现出减少的产量,表明对MIC以下抗生素暴露的反应不同.

科学领域:

微生物学微生物学
细菌病原体的产生
抗生素耐药性抗生素耐药性

背景情况:

抗生素的次最小抑制度 (sub-MICs) 可以调节细菌毒性因子.
Pseudomonas aeruginosa 是一种机会性病原体,以其产生酸盐的能力而闻名,酸盐是一种关键的毒性因子.

研究的目的:

为了研究 gentamicin 在亚MIC (0.5 MIC 和 0.25 MIC) 对 Pseudomonas aeruginosa 的临床分离物中阿尔金酸盐生产的影响.

主要方法:

通过微稀释来确定88 P. aeruginosa临床分离物中的 gentamicin 最低抑制度 (MIC).
使用碳醇方法评估酸盐生产.
使用PCR确认了酸盐基因的存在 (algD,algU).

主要成果:

所有的分离物都产生了酸盐,并具有algD和algu基因.
根他米辛子MIC显著增加了34个孤立 (38.6%) 和49个孤立 (55.7%) 的酸盐产量.
五个隔离物 (5.7%) 显示,在0.5MIC gentamicin时,酸盐产量减少,但在0.25MIC时,产量增加.

结论:

在亚MIC中,甘胺对P. aeruginosa的酸盐生产有多种影响.
需要进一步的研究来阐明这些差异性反应对MIC以下抗生素暴露的机制.

关键词:

阿尔基纳酸盐是一种酸盐.伪omonas aeruginosa 这种类型的病毒.gentamicins 是一种非常友好的药物.毒性因素是毒性的因素.

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